Objective To study the changes in the expressions of Wnt signaling gene in spinal cord at different times following spinal cord complete transection injury in rats. Methods Thirty healthy Wistar rats were randomly divided into 2 groups:the sham group (only laminectomy, EM>n/EM>=5) and the spinal cord injury (SCI) group (EM>n/EM>=25). The T11 spinal cord segment of adult rats was transected completely in the SCI group. The locomotor functional recovery of hind limbs was observed by grading the BassoBeattieBresnahan(BBB) scale. By using semiquantitative RT-PCR method, the expression of Wnt signaling gene in the injured tissues of spinal cord was detected. Results The hind limbs of rats were completely paraplyzed in the SCI group and their BBB scales were significantly lower than those in the sham group. In the SCI group, the expression of Wnt3a gene had a remarkable increase both on the rostral and the caudal sides at postoperative days 1, 3, 7; on the caudal side, it was higher than that on the rostral side at the same time and had a second increase at day 21 after the operation. The expression of Ngn1 was not detected. Hes1 mRNA was significantly decreased at day 1 after the operation, and increased at day 3 and then gradually decreased. Conclusion Wnt signaling may play an important role in the neural regeneratio