Objective To establish transgenic mice with nervous tissues specifically expressing green fluorescent protein and to make an animal model for the study of nervous system by fluorescent imaging. Methods The transgenic plasmid was constructed by inserting the EGFP gene under the control of PDGF Bchain promoter and transgenic C57BL/6J mice were established by microinjection method. The transgenic mice were genotyped by PCR. The total of 48 pairs of freezing sections of transgenic mice were prepared in the sagittal plane, and every two adjacent sections were observed under microscope and compared with HE staining and fluorescent imaging. Results One transgenic line with high levels of green fluorescent protein expression was detected from eight transgenic lines. Green fluorescent protein expression was observed in the tissues of cerebrum, hippocampus, thalamencephalon, cerebellum and brain stem. Conclusion A transgenic mouse line was established with nervous tissues specifically expressing green fhcore sunt protein. The results suggested that it will be an available animal model for the study of nervous tissues by fluorescent imaging.