荧光定量RT-PCR检测去下颌下腺大鼠睾丸annexin 5 mRNA和Bax mRNA的表达

王晨阳; 马百坤; 侯林; 徐会茹; 黄祝; 蒋超; 黄宇烽; 姚兵

解剖学报 ›› 2008, Vol. 39 ›› Issue (1) : 106-110.

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荧光定量RT-PCR检测去下颌下腺大鼠睾丸annexin 5 mRNA和Bax mRNA的表达

王晨阳^{1,2 ;马百坤^{1; 侯林^{1,2; 徐会茹^{1,2;黄祝^{1,2;蒋超^{1,2;黄宇烽^{1;姚兵^1*}}}}}}}

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THE EXPRESSIONS OF ANNEXIN 5 mRNA AND BAX mRNA WITH FQ-RT-PCR IN THE RAT TESTIS WITH SUBMANDIBULAR GLAND REMOVED

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Abstract

Objective To detect the expressions of annexin 5 mRNA and Bax mRNA in the rat testis after the submandibular gland was removed. Methods On days 14, 28 and 42 after the operation, the expression levels of annexin 5 and Bax mRNA in the rat testis were examined by fluorogenic quantitative RT-PCR. Results Fluorogenic quantitative RT-PCR showed that there was a 38.5% and 55.3% increase in the expression of annexin 5 mRNA and a 70.6% and 80.5% significant increase in the expression of Bax mRNA (P<0.05, P<0.01) in the rat testis on days 14 and 28 respectively. There was no significant change in the expressions of annexin 5 and Bax mRNA on day 42 compared with the control groups.Conclusion The removal of submandibular gland can significantly increase the expressions of annexin 5 and Bax mRNA in the rat testis on days 14 and 28. The expressions of annexin 5 and Bax mRNA goes back to the normal level on day 42

导出引用

王晨阳;马百坤;侯林;徐会茹;黄祝;蒋超;黄宇烽;姚兵. 荧光定量RT-PCR检测去下颌下腺大鼠睾丸annexin 5 mRNA和Bax mRNA的表达[J]. 解剖学报. 2008, 39(1): 106-110

THE EXPRESSIONS OF ANNEXIN 5 mRNA AND BAX mRNA WITH FQ-RT-PCR IN THE RAT TESTIS WITH SUBMANDIBULAR GLAND REMOVED[J]. Acta Anatomica Sinica. 2008, 39(1): 106-110

中图分类号： R339.2+1