Objective To correlate microanatomy of the Labbé vein with its venograms in order to provide a basis for the preservation of the Labbé veins in the supratentorial surgical approaches. Methods A total of 30 human cadavers (60 sides) and 61 patients (110 sides) were examined in this study. Each head of the cadavers were injected with bluecoloured latex via the superior sagittal sinus and internal jugular veins. The venograms of each patient were obtained from the venous phases of Digital Subtraction Angiography (DSA) (60 sides out of 36 patients) or Computed Tomographic Venography (CTV) (50 sides out of 25 patients). Results Based on the location of the dural entrance where a vein enters a dural sinus, the Labbé veins were divided into four groups: transverse sinus group, tentorial group, petrosal group and uppertransverse sinus group. In the transverse sinus and petrosal groups, the Labbé veins drained into transverse sinus directly,while indirectly in the tentorial and uppertransverse sinus groups, via the tentorium sinus or the uppertransverse sinus, which was a kind of meningeal veins running in the two layers of cerebral dura matter. The length of the meningeal veins was(10.0±7.2)mm (1.7-23.6mm). The Labbé veins were mainly located around the STP junction which is at the confluence of sigmoid sinus, transverse sinus and superior petrosal sinus. The distance between the dural entrance of the Labbé vein and STP junction was (16.8±10.2)mm (0-40.1mm). Compared with the observation in cadavers, there was no difference in DSA and CTV examinations.Conclusion The preoperative venogram is useful to design the individualized surgical approach for the preservation of the Labbé veins. In general, the medium supratentorial approach has the l