AcSDKP对TGF-β 1诱导的大鼠心脏成纤维细胞胶原蛋白合成及表达的调节作用

吴芳; 杨方; 王小君; 刘丽; 杨嫣; 王瑞; 马文东; 罗玲; 户万秘; 裴鑫; 张丽娟

解剖学报 ›› 2007, Vol. 38 ›› Issue (6) : 708-712.

论著

AcSDKP对TGF-β _{1诱导的大鼠心脏成纤维细胞胶原蛋白合成及表达的调节作用}

吴芳^{1，2; 杨方^{1*; 王小君^{1 ;刘丽^{1 ;杨嫣^{3;王瑞^{1 ;马文东^{1 ;罗玲^{1 ;户万秘^{1 ;裴鑫^{1 ;张丽娟¹}}}}}}}}}}

作者信息 +

EFFECT OF AcSDKP ON THE COLLAGEN SYNTHESIS AND EXPRESSION IN CULTURED RAT CARDIAC FIBROBLASTS MEDIATED BY TRANSFORMING GROWTH FACTOR BETA1

Author information +

文章历史 +

Abstract

Objective To investigate whether AcSDKP can inhibit collagen synthesis in cultured rat cardiac fibroblasts mediated by transforming growth factor beta1(TGF-βSUB>1/SUB>).Methods Neonatal rat cardiac fibroblasts were isolated. The synthesis of collagen was measured by SUP>3/SUP> Hproline incorporation assay. The expressions of type Ⅰ and Ⅲ collagen protein were detected by immunocytochemistry and Western blotting. Results SUP>3/SUP> Hproline content increased gradually (147.6±10.2， 229.2±16.4，427.0±40.6，454.8±26.1 CPM) and was 1.61， 2.50，4.66 and 4.97 times of control group(91.6±9.8 CPM) respectively in rat cardiac fibroblasts mediated by TGF-βSUB>1/SUB> concentration of 1μg/L, 2.5μg/L, 5μg/L and 7.5μg/L. Compared with TGF-βSUB>1/SUB> group (5μg/L), SUP>3/SUP> Hproline content decreased gradually(378.8±6.4，292.8±14.4，130.6±17.6 and 230.6±19.4 CPM) and was 88.7%, 68.6%， 30.6% and 54.0% of TGF-β1 group with increasing of AcSDKP concentration(10SUP>-10/SUP>mol/L, 5×10SUP>-10/SUP>mol/L, 10SUP>-9/SUP>mol/L, 10SUP>-8/SUP>mol/L). Compared with control group, the expression of collagen type Ⅰ and type Ⅲ in rat cardiac fibroblasts increased significantly in TGF-βSUB>1/SUB> group(5μg/L). Obsorbance values

导出引用

吴芳;杨方;王小君;刘丽 ;杨嫣;王瑞 ;马文东 ;罗玲;户万秘;裴鑫;张丽娟. AcSDKP对TGF-β _{1诱导的大鼠心脏成纤维细胞胶原蛋白合成及表达的调节作用}[J]. 解剖学报. 2007, 38(6): 708-712

EFFECT OF AcSDKP ON THE COLLAGEN SYNTHESIS AND EXPRESSION IN CULTURED RAT CARDIAC FIBROBLASTS MEDIATED BY TRANSFORMING GROWTH FACTOR BETA1[J]. Acta Anatomica Sinica. 2007, 38(6): 708-712

中图分类号： R542.2SUP>+/SUP>3