Objective To investigate the decreasing blood glucose effects of transplanting human insulinlike growth factor1(hIGF-1) engineering cells to diabetic model mice. Methods The hIGF-1 cDNA fragment was cloned into pAAV vector, then the viral particles were packaged with HEK293 incasing cells and the virus titer was identified with HT1080 cells. The marrow stromal cells were infected with high virus titer supernatant and transplanted into the diabetic model mice abdominal cavity. The effects of transplanting of virus infected marrow stromal cells on blood glucose of diabetic model mice were detected. Results The pAAV/ hIGF-1 plasmid was successfully constructed and the viral titer is up to 1012. The infection rate of marrow stromal cells was 60%. After the diabetic model mice were transplanted with bone marrow stromal cells that stably express hIGF-1 gene, the blood glucose decreased obviously (P0.05).Conclusion The marrow stromal cells that stably express hIGF-1 gene can obviously decrease blood glucose of diabetic model mice, which suggest that hIGF-1 gene can b