RNA干扰抑制Nogo-A基因表达对PC12细胞多巴胺释放的影响

熊南翔; 蒲建章; 赵洪洋; 苏群; 姚东晓; 冯军

解剖学报 ›› 2007, Vol. 38 ›› Issue (5) : 537-541.

论著

RNA干扰抑制Nogo-A基因表达对PC12细胞多巴胺释放的影响

熊南翔^{1 ;蒲建章^{1; 赵洪洋^{1* ; 苏群^{2 ;姚东晓^{1; 冯军^1}}}}}

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INHIBITION OF NOGO-A GENE EXPRESSION BY RNA AND ITS EFFECTS ON DOPAMINE RELEASE IN PC12 CELLS

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Abstract

Objective To study the effect of Nogo-A gene silence on dopamine release in PC12 cells. Methods The small hairpin RNA(shRNA) eukaryotic expression vector targeting Nogo-A gene was constructed and transfected into cultured PC12 cells by lipofecamine2000. The inhibiting effect was detected by semiquantitative reverse transcription(PCR) and Western blotting analysis. The dopamine release was detected by HPLC(high performance liquid chromatography) after transfection. Results The Nogo-A gene expression was inhibited and the dopamine release decresed significantly 48 hours after transfection.Conclusion The reasults suggest that Nogo-A be involved in the mechanism of dopamine realese in PC12 cells.