大鼠体神经-内脏神经吻合再生后脊髓前角异体神经元的分离和凝集素的表达变化及作用

海波; 刘宇; 陈朝晖; 李伟; 钟伟; 肖传国

解剖学报 ›› 2007, Vol. 38 ›› Issue (5) : 515-519.

论著

大鼠体神经-内脏神经吻合再生后脊髓前角异体神经元的分离和凝集素的表达变化及作用

海波^{1;刘宇^{2; 陈朝晖^{1; 李伟^{1 ; 钟伟^{1 ;肖传国^1*}}}}}

作者信息 +

ISOLATION OF FOREIGN NEURONS IN CORNU ANTERIUS MEDULLAE SPINALIS AFTER ARTIFICAL SOMATICAUTONOMIC NERVE ANASTOMOSIS OF RATS AND STUDY OF EXPRESSION CHANGES OF AGRIN

Author information +

文章历史 +

Abstract

Objective To study the changes of agrin expression in spinal cord motor neurons after somaticautonomic ventral root (VR) anastomosis and somaticsomatic VR anastomosis, and the factors related to the synapse formation of foreign nerve regeneration. Methods Artifical somaticautomomic reflex pathway and somatic nerve selfanastomosis were established in rats. Retrograde tracing was carried out to label the motor neurons, and the neurons were isolated by flow cytometry. Realtime PCR was used to investigate the agrin mRNA expression of the neurons.Anterograde tracing and immunofluorescence technique were employed to label the new synapses of pelvic ganglia (PG). Results After 4 months of surgery, the somatic nerve can regenerate into PG successfully and form new synapses observed under laser scanning confocal microscope. The neurons involved in foreign nerve regeneration can be isolated successfully by retrograde tracing and flow cytometry.The expression of agrin in LSUB>4/SUB>-LSUB>6/SUB> VRs anastomosis group was lower than that of the control group of LSUB>4/SUB>-LSUB>4 /SUB>VR self-anastomosis (P<0.05) and the normal group (P<0.05).Conclusion The somatic nerve could regenerate into PG and form new synapses.The differences of agrin expression

导出引用

海波;刘宇;陈朝晖;李伟;钟伟;肖传国. 大鼠体神经-内脏神经吻合再生后脊髓前角异体神经元的分离和凝集素的表达变化及作用[J]. 解剖学报. 2007, 38(5): 515-519

ISOLATION OF FOREIGN NEURONS IN CORNU ANTERIUS MEDULLAE SPINALIS AFTER ARTIFICAL SOMATICAUTONOMIC NERVE ANASTOMOSIS OF RATS AND STUDY OF EXPRESSION CHANGES OF AGRIN[J]. Acta Anatomica Sinica. 2007, 38(5): 515-519

中图分类号： R322.85