Objective To investigate the expression of δ-Catenin in the cerebellum of ovariectomed rat with estrogen replacement and the estrogen effect on δ-Catenin expression in the cerebellum of the rats with ovariectome. Methods The rats were divided into normal group, sham operation group, estrogen replacement treatment group and placebo group. Then the rats were artificially injected estradiol or placebo after ovariectome. The expression of δ-Catenin in the cerebellum was detected by immunohistochemical method and was quantitated by Western blotting analyses. Results Immunohistochemical method and Western blotting analyses both displayed the expression of δ-Catenin in all the groups, while the expression in placebo group was the lowest among the four groups, the expression of δ-Catenin in the groups of rats with estrogen replacement treatment and sham operation was significantly higher than that of placebo group.Conclusion The estrogen may upregulate the expres