Objective Study the function of p21activated kinase 2 in Xenopus oocyte cytokinesis. Methods Xenopus oocyte was used as a model, and MPF activity and PAK2 phosphorylation status were observed during oocyte maturation. The specific inhibitor of PAK2 activity, PAK2NT (PAK2Nterminal) was used to microinject into Xenopus oocyte. Under fluorescent microscope germinal vesicle breakdown was observed during cytokinesis. To further observe the changes of Factin and spindle, laser scanning confocal microscopy with timelapse method was employed. Results Occurrences of germinal vesicle breakdown in control oocytes was similar to those oocytes injected with PAK2NT mRNA, but no cytokinesis was observed in oocytes injected with PAK2N