Objective To investigate the milieu-dependent differentiation of primordial germ cells(PEGs) in the acute damaged liver microenviroment. Methods After PGCs were cultured and proliferated, these cells were labelled with 5-bromo-2-deoxyuridine(BrdU), then transplanted into the acute damaged liver by CCl\-4 through tail vein. Two and four weeks later, the liver was extracted and 10μm -cryostat continuous sections were obtained. The existing and differentiation of the transplanted cells were identified by immunohistochemistry, immunofluorescence double staining and histochemistry for BrdU and hepatic-specific ALB, and the glycogen. Results Transplanted PGCs were found to be incorporated into the acute damaged liver and differentiated into hepatocytes, compensating for acute liver failure.Conclusion PGCs can be induced to differentiate into hepatocytes in the acute damaged liver microenvironment, and can be used for cellular hepatoplasty to treat severe liver disease.