低剂量棉酚和甾体激素联合用药对精母细胞凋亡和支持细胞吞噬作用的影响

常青; 钱晓菁; 许增禄; 张成侠

doi:10.3969/j.issn.0529.1356.2010.02.02２

解剖学报 ›› 2010, Vol. 41 ›› Issue (2) : 276-280. DOI: 10.3969/j.issn.0529.1356.2010.02.02２

论著

低剂量棉酚和甾体激素联合用药对精母细胞凋亡和支持细胞吞噬作用的影响

常青; 钱晓菁 ;许增禄*; 张成侠

作者信息 +

Effects of combined administration of low dose gossypol with steroid hormones on spermatocyte apoptosis and phagocytosis of sertoli cells

Author information +

文章历史 +

Abstract

Objective To investigate effects of combined regimen of low dose gossypol with steroid hormones on apoptosis of spermatocyte and phagocytosis of sertoli cells. Methods Adult male rats were divided into four groups randomly, group GH: rats were fed orally with gossypol(GA) ［12.5mg / (kgI>&#/I>8226;d) ］ and desogestrel(DSG)［125μg/(kgI>&#/I>8226;d) ］/ethinyloestradil(E)［25μg/ (kgI>&#/I>8226;d) ］/testosterone undecanoate(TU)［100mg/(kgI>&#/I>8226;d) ］; group G: a single does of GA［12.5mg/(kgI>&#/I>8226;d ) ］ was given; group H: DSG［125μg/(kgI>&#/I>8226;d) ］/E［25μg/(kgI>&#/I>8226;d) ］/TU［100mg/(kgI>&#/I>8226;d) ］ were administered; group C: rats only received vehicle(1% methyl cellulose). Testes from all the rats were removed at 4, 6 and 8 weeks after treatment for counting of spermatocyte and round spermatid using stereological assay, and for detecting apoptosis of spermatocyte and phagocytosis of sertoli cell by TUNEL and oil red O staining respectively. Results In GH group, the number of spermatocyte and round sperm

导出引用

常青;钱晓菁;许增禄;张成侠. 低剂量棉酚和甾体激素联合用药对精母细胞凋亡和支持细胞吞噬作用的影响[J]. 解剖学报. 2010, 41(2): 276-280 https://doi.org/10.3969/j.issn.0529.1356.2010.02.02２

Effects of combined administration of low dose gossypol with steroid hormones on spermatocyte apoptosis and phagocytosis of sertoli cells[J]. Acta Anatomica Sinica. 2010, 41(2): 276-280 https://doi.org/10.3969/j.issn.0529.1356.2010.02.02２

中图分类号： R979.2