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骨髓间充质干细胞的组织亲和性与脑片移植
Tissue affinity of bone marrow mesenchymal stem cell and the therapeutic transplantation for neurodegenerative diseases
目的 检测骨髓间充质干细胞的组织亲和性、时间亲和性以及移植治疗神经元退行性疾病的可行性。 方法 应用组织细胞共培养和免疫细胞化学的方法观察骨髓间充质干细胞在不同组织的微环境下的黏附和向神经细胞的分化情况。 结果 与肝片、肺片相比，骨髓间充质干细胞在海马脑片上的黏附力最强；骨髓间充质干细胞在生后7d（P7）小鼠脑片上的黏附力最强，并随着年龄的增长呈逐渐递减的趋势；骨髓间充质干细胞在正常野生型小鼠和阿尔茨海默病小鼠脑片上都能向神经细胞分化，并且两者无显著差异。 结论 骨髓间充质干细胞更适宜用于脑部疾病的治疗，并且年龄越小移植效果越好。
Objective To study the affinity of bone marreow mesenchymal stem cells (BMSCs) to various tissues and the possibility for therapeutic transplantation to cure neurodegenerative diseases with BMSCs. Methods The hippocampal slices, liver slices and lung slices were co-cultured with BMSCs to investigate BMSCs affinity to the tissues above, and immunohistochemistry (NeuN and GFAP) was carried out to understand the differentiation of BMSCs into neurons and glial cells under microenvironment of hippocampal slices, liver slices and lung slices. Results BMSCs had a highest affinity with hippocampal slices in contrast with the slices of liver and lung. The BMSCs’ temporal affinity on hippocampal slice was observed as well. At P7, BMSCs had the highest affinity with hippocampus, but there was a time-related decrease afterward. Differentiation of BMSCs occurred on hippocampal slices of both WT and Tg mice. Under the induction of hippocampal microenvironment, BMSCs differentiated into nervous cells and glial cells, but there was no significance between WT and Tg mouse. Conclusion BMSCs may provide an opportunity for the therapeutic transplantation to cure the neurodegenerative diseases and brain injuries. The time-related decrease of cell adhesion to hippocampus shows that the early stem cell transplantation is necessary.
骨髓间充质干细胞 / 亲和性 / 神经元退行性疾病 / 免疫组织化学 / 小鼠
Bone marrow mesenchymal stem cell / Affinity / Neurodegenerative disease / Immunohistochemistry / Mouse
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中国国家自然科学基金;中国国家自然科学基金;中国国家自然科学基金
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