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Tg2576小鼠海马发育过程中小胶质细胞和血管的变化
Alteration of microglia and vasculature in the developing Tg2576 transgenic mouse
目的 探讨Tg2576转基因小鼠发育过程中海马CA1区小胶质细胞增殖和血管变化的规律。方法 取不同发育时间(P0、P7、P30、P180、P360) Tg2576转基因模型鼠与同时间点野生鼠，通过应用免疫组织化学、TUNEL、墨汁灌注、RT-PCR和透射电镜等方法研究海马发育过程中小胶质细胞和血管的变化。结果 随着小鼠的生长发育，P180后转基因组海马CA1区小胶质细胞密度和血管体密度高于对照组小鼠，RT-PCR结果显示，P360时转基因组海马CA1区小胶质细胞更多处于激活状态。 结论 小胶质细胞与血管改变的共同作用加重了阿尔茨海默病。
Objective Our aim is to study the alteration of microglia and vasculature in the developing hippocampus of Tg2576 transgenic mice. Methods Tg2576 transgenic mice from postnatal day 0 (P0) to P360 were used for Iba1 and NeuN immunohistochemistry, TdT-mediated dUTP nick-end labeling (TUNEL), ink perfusion and RT-PCR analysis. Results From P180, the density of Iba1-positive cells in CA1 area of the Tg2576 transgenic mouse was significantly higher than that of the wild type mouse. The vasculature (volumetric density) of the transgenic mouse at P360 was significantly less than in the wild-type mouse (P<0.01). RT-PCR results also showed that the activity of microglia was enhanced in the AD model. Conclusion The onset and development of Alzheimer disease is correlated to the alteration of microglia and vasculature in hippocampus.
小胶质细胞 / 激活 / 阿尔茨海默病 / 淀粉样前体蛋白 / 免疫荧光 / 小鼠
Microglia / Activation / Alzheimer’s disease / Amyloid precursor protein / Immunofluorescence / Mouse
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中国国家自然科学基金面上项目
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