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脂多糖对巨噬细胞铁代谢的影响
Effect of lipopolysaccharide on the iron metabolism in macrophages
目的 检测二价金属离子转运体（DMT1）和金属转运蛋白（FPN1）在原代培养巨噬细胞中的分布及脂多糖（LPS）对巨噬细胞铁代谢的影响。 方法 采用原代细胞培养、MTT显色、细胞化学染色和细胞免疫荧光等方法检测LPS对原代培养的巨噬细胞活性的作用及对DMT1和FPN1分布的影响。 结果 不带铁反应元件的二价金属离子转运体（DMT1-IRE）主要在细胞核中表达，在细胞质中分布较少。带铁反应元件的二价金属离子转运体（DMT1 +IRE）主要分布于细胞质中，可以将吞噬小体中的铁向细胞质中转运。FPN1在巨噬细胞的细胞质和细胞膜均有表达，但主要分布于细胞质。结论 巨噬细胞吞噬衰老的红细胞以后，其中的亚铁血红素在细胞质中分解，FPN1可能介导了亚铁血红素分解释放出来的铁在巨噬细胞质中的转运过程。LPS在低浓度时有促进巨噬细胞生长的作用，LPS 浓度为10-5μg/L时达到高峰，随着浓度的增加，又开始抑制细胞生长。
Objective To study the effect of lipopolysaccharide(LPS) on the activity of primary cultured macrophages and the distribution of divalent metal transporter 1 (DMT1) and ferroportin 1 (FPN1). Methods Primary cell culture, MTT chromotest, cytochemistry chromotest and cell immunofluorescence techniques were used in this work.
Results DMT1 was mainly distributed in the cytoplasm, which illuminates that DMT1 mediates the macrophage intracellular transit of iron from phagolysosome to cytoplasm. FPN1 was distributed in the cytoplasm and membrane, and the cytoplasm was the main site of FPN1 distribution in macrophages. Conclusion Iron liberation from heme inside the phagolysosome occurs after erythrophagocytosis and it is possible that FPN1 mediates intracellular transit of iron released by heme catabolism. The study found that LPS promoted the cell growth and this effect was reached to the peak in the 10-5 μg/L LPS group, but the cell growth was blocked with the increase of LPS concentration.
二价金属离子转运体 / 金属转运蛋白 / 原代培养 / 巨噬细胞 / 小鼠
Divalent metal transporter 1 / Ferroportin 1 / Primary culture / Macrophage / Mouse
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