两种帕金森病大鼠糖脂代谢模型的比较

孟祥志 郑瑞攀 张迎 乔明亮 景鹏 高艳*

doi:10.3969/j.issn.0529-1356.2014.05.004

解剖学报 ›› 2014, Vol. 45 ›› Issue (5) : 605-609. DOI: 10.3969/j.issn.0529-1356.2014.05.004

神经生物学

两种帕金森病大鼠糖脂代谢模型的比较

孟祥志¹ 郑瑞攀¹张迎¹ 乔明亮² 景鹏¹ 高艳^1*

作者信息 +

Comparison of glucose and lipid metabolism in two types of Parkinson’s disease rat models

MENG Xiang-zhi¹ ZHENG Rui-pan¹ZHANG Ying¹QIAO Ming-liang²JING Peng¹ GAO Yan ^1*

Author information +

文章历史 +

摘要

目的筛选合并代谢异常的帕金森病模型，为后续的相关实验研究提供理想的动物模型。方法 SD雄性大鼠62只，随机分为损毁前脑内侧束（MFB）模型组、损毁MFB假手术组、损毁纹状体模型组和损毁纹状体假手术组。模型制做成功后进行旋转实验，两组大鼠中分别选出成功模型，并分别对两组成功的PD模型大鼠的摄食、体重、血糖和腹腔内脂肪组织进行检测。观察两组模型大鼠上述代谢指标的变化。结果损毁MFB较损毁纹状体制作帕金森病模型的成功率高(70.97% 比38.71%)。与各假手术组大鼠相比，MFB模型大鼠既出现体重明显的减轻［(218.1±13.99)g 比（252.7±10.1）g， P<0.05］，腹腔注射葡萄糖后15min和30min血糖出现了明显升高，并且肾周白色脂肪组织明显减少(左右两侧)。纹状体模型大鼠仅饮食减少［(13.95±0.25)g 比（20.23±0.86）g，P<0.001］和腹腔注射葡萄糖后15min、30min和60min时间点血糖升高。结论损毁MFB的PD模型大鼠无论模型成功率还是代谢相关指标都较损毁纹状体的PD模型更接近糖脂代谢异常的PD患者。

Abstract

Objective To select an ideal Parkinson’s disease (PD) animal model with metabolic abnormalities for subsequent experimental studies. Methods A total of 62 Sprague-Dawley male rats were randomly divided into four groups: damaged medial forebrain bundle (MFB) model group, damaged medial forebrain bundle (MFB) sham group, damaged Striatum model group and damaged Striatum sham group. After detecting the rotation experiment, successful model rats of two groups were selected to detect the changes of food intake, body weight, blood glucose and intra-abdominal adipose tissue. Results It was easier to produce a PD model by destroying MFB than striatum. Compared with sham-operated rats, MFB model rats showed significant abnormality both in reduction of body weight ［(218.1±13.99)g vs (252.7±10.1)g, P<0.05］ and high blood glucose appeared at 15min and 30min after introperitoneal glucose tolerance test (IPGTT). Their perirenal white adipose tissue was significantly reduced (both left and right side). Striatum model rats only appeared decreased food intake ［(13.95±0.25)g vs (20.23±0.86)g, P<0.001］ and impaired glucose regulation at 15min, 30min and 60min after IPGTT. Their body weight and adipose tissue did not change significantly. Conclusion No matter in the success rate or metabolismrelated indicators, MFB damaged rat model of PD is more suitable to study PD patients with abnormal lipid metabolism compared with Striatum rat model.

导出引用

孟祥志郑瑞攀张迎乔明亮景鹏高艳*. 两种帕金森病大鼠糖脂代谢模型的比较[J]. 解剖学报. 2014, 45(5): 605-609 https://doi.org/10.3969/j.issn.0529-1356.2014.05.004

MENG Xiang-zhi ZHENG Rui-pan ZHANG Ying QIAO Ming-liang JING Peng GAO Yan*. Comparison of glucose and lipid metabolism in two types of Parkinson’s disease rat models[J]. Acta Anatomica Sinica. 2014, 45(5): 605-609 https://doi.org/10.3969/j.issn.0529-1356.2014.05.004

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