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钠尿肽受体A在小鼠视网膜发育过程中的表达
Expression of natriuretic peptide receptor A in the developing retina of the mouse
目的 检测钠尿肽受体(NPR)在不同年龄小鼠视网膜内的表达，探讨其在视网膜发育过程中的作用。方法 收集从受孕16日(E16)到出生90日（P90）小鼠眼球标本共127只，对NPR-A进行免疫荧光检测。结果 NPR-A广泛存在于视网膜神经元中，例如，在外核层，NPR-A于P7开始高表达在视锥、视杆细胞内、外突起上，于P14减弱，P30之后持续稳定弱表达；在内核层，从P7开始NPR-A持续弱表达在双极细胞的突起中，而在水平细胞中未见NPR-A表达；在神经节细胞层，NPR-A于E16开始高表达在神经节细胞胞体中，P14明显减弱，而在神经纤维层，即神经节细胞的轴突中，NPR-A从胚胎期至成年持续高表达；在外网状层和内网状层，NPR-A于P14均高表达，但于P30之后逐渐减弱。此外，NPR-A还广泛的存在于Müller细胞的突起中。 结论 NPR-A参与了视网膜的发育，可能是小鼠视网膜神经元发育过程中的关键分子，并对Müller细胞的功能活动起着重要的调节作用。
Objective Our purpose is to investigate the expression of natriuretic peptide receptor A (NPR-A) in the retina and to understand the NPR-A’s functions during the mouse development. Methods Mice eyes were harvested from E16 (embryonic day 16) to P90 (postnatal day 90). Total of 127 eyes were used in the study. Immunohistochemistries of NPR-A were carried out. Results During development, NPR-A was widely expressed in the retinal neurons. In the outer nuclear layer, NPR-A began to appear in the inner and outer projections of cone and rod cells at P7, but decreased at P14. From P30 afterward, it continued to express weakly. In the inner nuclear layer, NPR-A expressed in the dendrites of bipolar cells weakly from P7 to adulthood, whereas no expression in horizontal cells. In the ganglion cell layer, NPR-A started highly to express in the ganglion cell bodies at E16, and in the meantime, in the nerve fiber layer, ganglion cell axons, NPR-A was expressed highly from embryonic to adult. In the inner and outer plexiform layers, NPR-A was highly expressed at P14, but decreased gradually after P30. In addition, NPR-A also widely expressed in the inner protrusions of Müller cells. Conclusion NPR-A participates in the development of the retina, and may be the key molecule in the developing retinal neurons. Moreover, it plays an important regulatory role in the functional activity of Müller cells.
视网膜 / 发育 / 钠尿肽受体A / 免疫组织化学 / 小鼠
Retina / Development / Natriuretic peptide receptor A / Immunohistochemistry / Mouse
［1］ Potter LR, Yoder AR, Flora DR, et al. Natriuretic peptiods: their structures,receptors, physiologic functions and therapeutic applications ［J］. Handb Exp Pharmacol, 2009, 191(10):341-366.
［2］Stone RA, Glembotski CC. Immunoactive natriuretic peptide in the rat eye: molecular forms in anterior uvea and retina ［J］. Biochem Biophys Res Commun, 1986,134(2):1022-1028.
［3］Fernandez-Durango R, Trivino A, Ramirez JM, et al. Immunoreac-tive atrial natriuretic factor in aqueous humor: its concentration is increased with high intraocular pressure in rabbit eyes ［J］. Vision Res, 1990, 30(9):1305-1310.
［4］Kong WF, Zhou J, Jin HX, et al. Lamination of mouse retina and the neural stem cell proliferation and differentiation［J］. Acta Anatomica Sinica, 2013, 44(1): 13-18.(in Chinese)
孔维芳, 周洁, 金海啸，等.小鼠视网膜片层化及神经干细胞的增殖与分化［J］. 解剖学报, 2013, 44(1):13-18.
［5］Gwon JS, Chun MH, Kang WS. Regulatory expression and cellular localization of doublecortin in the rat retina following ischemia-reperfusion injury［J］. Animal Cells and Systems, 2011, 15(2): 155-159.
［6］Xue LP, Lu J, Cao Q, et al. Müller glial cells express nestin coupled with glial fibrillary acidic protein in experimentally induced glaucoma in the rat retina［J］. Neuroscience, 2006, 139(2): 723-732.
［7］Wohl SG, Schmeer CW, Isenmann S. Neurogenic potential of stem/progenitor-like cells in the adult mammalian eye［J］. Prog Retin Eye Res, 2012, 31(3):213-242.
［8］Fernandez-Durango R, Sanchez D, Gutkowska J, et al. Identifica-tion and characterization of atrial natriuretic factor receptors in the rat retina［J］. Life Sci, 1989, 44(24):1837-1846.
［9］Reese BE. Development of the retina and optic pathway［J］. Vision Res, 2011, 51(7): 613-632.
［10］Cao LH, Yang XL. Natriuretic peptide receptor-A is functionally expressed on bullfrog retinal Müller cells［J］. Brain Res Bull, 2007, 71(4): 410-415.
［11］Dixon DB, Copenhagen DR. Metabotropic glutamate receptor-mediated suppression of an inward rectifier current is linked via a cGMP cascade［J］. J Neurosci, 1997, 17(23):8945-8954.
［12］Cao LH. Modulation of activity of retinal bipolar cell by brain natriuretic peptide ［D］. Fudan University, 2007: 26-40. (in Chinese)
曹丽慧. 钠尿肽对视网膜双极细胞活动的调制［D］. 复旦大学, 2007: 26-40.
［13］Davis DM, Dyer MA. Retinal progenitor cells, differentiation, and barriers to cell cycle reentry［J］. Curr Top Dev Biol, 2010, 93(8):175-188.
［14］Yuan ShJ, Hu JJ, Ding WL, et al. Changes of expression of postsynaptic density protein 95 and synaptic parameters in the diabetic rat retina ［J］. Acta Anatomica Sinica, 2012, 43(6): 792-795.(in Chinese)
原淑娟,胡金家,丁文龙,等. 糖尿病大鼠视网膜突触后致密物蛋白质95的蛋白表达及突触超微结构的改变［J］. 解剖学报, 2012, 43(6): 792-795.
［15］Fischer AJ. Muller glia, vision-guided ocular growth, retinal stem cells, and a little serendipity: the cogan lecture［J］. Invest Ophthalmol Vis Sci, 2011, 52(10):7705-7710.
［16］Robel S, Berninger B, Gtz M. The stem cell potential of glia: lessons from reactive gliosis［J］. Nat Rev Neurosci, 2011, 12(2): 88-104.
［17］Fischer AJ, Reh TA. Müller glias are a potential source of neural regeneration in the postnatal chicken retina［J］. Nat Neurosci, 2001, 4(3):247-252.
［18］Lelièvre V, Pineau N, Hu Z, et al. Proliferative actions of natriuretic peptides on neuroblastoma cells involvement of guanylyl cyclase and non-guanylyl cyclase pathways［J］. J Biol Chem, 2001, 276(47): 43668-43676.
［19］S ánchez-López A, Cuadros MA, Calvente R, et al. Radial migration of developing microglial cells in quail retina: a confocal microscopy study［J］. Glia, 2004, 46(3):261-273.
［20］Derouiche A, Rauen T. Coincidence of L-glutamate/L-aspartate transporter (GLAST) and glutamine synthetase (GS) immunoreactions in retinal glia: evidence for coupling of GLAST and GS in transmitter clearance［J］. J Neurosci Res, 1995, 42(1): 131-143.
［21］Krishnan G1, Chatterjee N. Detergent resistant membrane fractions are involved in calcium signaling in Müller glial cells of retina［J］. Int J Biochem Cell Biol, 2013, 45(8):1758-1766.
［22］Tout S, Chan-Ling T, Holl?nder H, et al. The role of Müller cells in the formation of the blood-retinal barrier［J］. Neuroscience, 1993, 55(1): 291-301.
［23］Dahrouj M, Alsarraf O, Liu Y, et al. C-type natriuretic peptide protects the retinal pigment epithelium against advanced glycation end product-induced barrier dysfunction［J］. J Pharmacol Exp Ther, 2013, 344(1): 96-102.
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