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大鼠下颌下腺卵泡刺激素及其受体的原位杂交及核心片段的序列分析
In situhybridization and analysis of core sequence of follicle-stimulating hormone and its receptor in rats submaxillary
目的 探讨大鼠下颌下腺组织中是否表达卵泡刺激素(FSH)及其受体(FSHR)，为进一步研究FSH对下颌下腺的功能调节提供理论依据。方法 正常SD大鼠20只，体质量(200±20)g，腹腔麻醉后切取下颌下腺，采用原位杂交方法对FSH及FSHR进行细胞定位，探讨大鼠下颌下腺是否存在FSH及其受体mRNA，从下颌下腺组织中分别提取总RNA,运用RT-PCR获得FSH及其受体基因的cDNA核心序列，并对其序列进行分析。 结果 大鼠下颌下腺浆液性腺泡及颗粒曲管上皮细胞含有FSH和FSHR mRNA杂交信号，信号物质分布于胞质内，胞核呈阴性反应。从大鼠下颌下腺组织中扩增的FSH及FSHR基因的特异性条带分别为193bp和413bp。 结论 大鼠下颌下腺浆液性腺泡及颗粒曲管上皮细胞能合成卵泡刺激素及其受体，进一步说明下颌下腺是FSH作用的靶器官。
Objective To explore if the expression of follicle-stimulating hormone (FSH) and its receptor (FSHR) exist in the rat submandibular gland tissue, which may provide a theoretic evidence for further studying on adjusting function of the FSH in submaxillary gland. Methods Twenty normal SD rats, with a mean body weight of (200±20)g, were adopted to collect out the submandibular gland after abdominal cavity anesthesia. The method ofin situ hybridization was employed on cellular localization of FSH and FSHR. RT-PCR was used to detect if FSH and its receptor mRNA exist in the rat submandibular gland. RNA was extracted from the submandibular gland, then FSH and the core cDNA of its receptor gene were obtained with reverse transcription polymerase chain reaction and analyzed on its sequence. Results The epithelial cells of serous acinus and granular convoluted tubule in submaxillary gland of rats had FSH and FSHR mRNA hybridized signals, which were detected in cytoplasm with negative nuclei. Specific bands of FSH cDNA and FSHR cDNA were identical to be 193bp and 413bp respectively. Conclusion FSH and FSHR can be synthesized by the epithelial cells of serous acinus and granular convoluted tubule in submaxillary gland of rats, thus this study further proves that the submandibular gland is the target organ of FSH.
卵泡刺激素 / 卵泡刺激素受体 / 下颌下腺 / 原位杂交 / 反转录-聚合酶链反应 / 大鼠
Follicle-stimulating hormone / Follicle-stimulating hormone receptor / Submaxillary gland / In situ hybridization / RT-PCR / Rat
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卵泡刺激素在消化系统的表达及功能研究
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