神经生长因子促进海马胆碱能神经再生过程中Lhx8的表达

李浩明 朱培培 金国华* 施金洪 邹琳清 田美玲 衣昕 秦建兵

doi:10.3969/j.issn.0529-1356.2014.04.001

解剖学报 ›› 2014, Vol. 45 ›› Issue (4) : 441-445. DOI: 10.3969/j.issn.0529-1356.2014.04.001

神经生物学

神经生长因子促进海马胆碱能神经再生过程中Lhx8的表达

李浩明朱培培金国华* 施金洪邹琳清田美玲衣昕秦建兵

作者信息 +

Dynamic expression of Lhx8 in nerve growth factor induced hippocampal neuroregeneration

LI Hao-ming ZHU Pei-pei JIN Guo-hua* SHI Jin-hong ZOU Lin-qing TIAN Mei-ling YI Xin QIN Jian-bing

Author information +

文章历史 +

摘要

目的探讨神经生长因子（NGF）促进海马胆碱能神经再生与同源盒基因Lhx8之间的关系。方法 SD大鼠72只，在制备切割右侧穹隆海马伞模型和NGF侧脑室注射的基础上，分为对照组、切割组、NGF组、切割联合NGF组；Real-time PCR和Western blotting分别检测各组动物海马中Lhx8蛋白的表达变化；免疫荧光技术检测各组动物海马齿状回颗粒下层中胆碱乙酰转移酶(ChAT)/Lhx8双标细胞数。结果切割组和NGF组中Lhx8蛋白的表达量较对照组增高，切割联合NGF组增高更为明显，且Lhx8基因表达也增高；NGF组和切割联合NGF组中海马齿状回颗粒下层(SGZ)中的ChAT/Lhx8双标细胞数也较对照组和切割组明显增多。结论侧脑室注射NGF促进海马胆碱能神经再生与Lhx8的高表达有关。

Abstract

Objective To investigate the relationship between the nerve growth factor (NGF) induced hippocampal neuroregeneration and homeobox gene Lhx8.Methods Seventy-two SD rats were divided into control group, transected group, NGF group, transected combined with NGF group after right fimbria-fornix transection and NGF intracerebroventricular injection. Real-time PCR and Western blotting were applied to detect the gene and protein expression of Lhx8 in each group. The choline acetyltransferase (ChAT)/Lhx8 double labeled cells in subgranular zone (SGZ) of hippocampus in each group were detected by immunofluorescence. Results The expression of Lhx8 gene and protein in the transected, NGF group and especially in the transected combined with NGF group was obviously higher than in the control group. The number of ChAT/Lhx8 double labeled cells in the NGF group and the transected combined with NGF group was obviously more than in the control group and transected group. Conclusion The hippocampal neuroregeneration which induced by NGF intracerebroventricular injection was associated with the higher expression of Lhx8.

导出引用

李浩明朱培培金国华* 施金洪邹琳清田美玲衣昕秦建兵. 神经生长因子促进海马胆碱能神经再生过程中Lhx8的表达[J]. 解剖学报. 2014, 45(4): 441-445 https://doi.org/10.3969/j.issn.0529-1356.2014.04.001

LI Hao-ming ZHU Pei-pei JIN Guo-hua* SHI Jin-hong ZOU Lin-qing TIAN Mei-ling YI Xin QIN Jian-bing. Dynamic expression of Lhx8 in nerve growth factor induced hippocampal neuroregeneration[J]. Acta Anatomica Sinica. 2014, 45(4): 441-445 https://doi.org/10.3969/j.issn.0529-1356.2014.04.001

参考文献

［1］Eriksson PS，Perfilieva E，Bjork-Eriksson T，et al. Neurogenesis in the adult human hippocampus［J］.Nat Med，1998，4（11）：1313-1317.
［2］Gubert F，Zaverucha-do-Valle C，Pimentel-Coelho PM，et al.Radial glia-like cells persist in the adult rat brain［J］.Brain Res，2009，1258 (1)：43-52.
［3］Dong ChM, Jin GH, Qin JB, et al. The effect of NGF on the proliferation and differentiation of endogenous NSCs in the hippocampus into neurons after fimbria fornix transection［J］. Acta Anatomica Sinica, 2007, 38 (06): 642-646.(in Chinese)
董传明，金国华，秦建兵，等.神经生长因子对穹隆海马伞切割后海马自体神经干细胞增殖和向神经元分化的影响［J］.解剖学报，2007, 38（06）：642-646.
［4］Xiong Y, Tian ML, Qin JB, et al. Effects of NGF on inducing the rat embryonic neural stem cells derived from cortex and septal region to differentiate into neurons and AChE positive neurons in vitro［J］. Journal of Nantong University (Medical Sciences), 2007, 27 (1): 11-13. (in Chinese)
熊咏，田美玲，秦建兵，等.NGF诱导大鼠胚脑皮层和隔区神经干细胞向神经元和AChE阳性神经元分化的作用［J］.南通大学学报(医学版)，2007, 27（1）：11-13. (in Chinese)
［5］Zhang X，Jin G，Tian M，et al. The denervated hippocampus provides proper microenvironment for the survival and differentiation of neural progenitors［J］.Neurosci Lett，2007，414（2）：115-120.
［6］Shi J，Li H，Jin G，et al. Lhx8 promote differentiation of hippocampal neural stem/progenitor cells into cholinergic neurons in vitro［J］.In Vitro Cell Dev Biol Anim，2012，48（10）：603-609.
［7］Zhao Y，Marin O，Hermesz E，et al.The LIM-homeobox gene Lhx8 is required for the development of many cholinergic neurons in the mouse forebrain［J］.Proc Natl Acad Sci USA，2003，100（15）：9005-9010.
［8］Mori T，Yuxing Z，Takaki H，et al. The LIM homeobox gene, L3/Lhx8, is necessary for proper development of basal forebrain cholinergic neurons［J］.Eur J Neurosci，2004，19（12）：3129-3141.
［9］Fragkouli A，van Wijk NV，Lopes R，et al. LIM homeodomain transcription factor-dependent specification of bipotential MGE progenitors into cholinergic and GABAergic striatal interneurons［J］.Development，2009，136（22）：3841-3851.
［10］Flandin P，Zhao Y，Vogt D，et al. Lhx6 and Lhx8 coordinately induce neuronal expression of Shh that controls the generation of interneuron progenitors［J］.Neuron，2011，70（5）：939-950.
［11］Balu DT，Lucki I.Adult hippocampal neurogenesis: regulation, functional implications, and contribution to disease pathology［J］.Neurosci Biobehav Rev，2009，33（3）：232-252.
［12］Bruel-Jungerman E，Rampon C，Laroche S.Adult hippocampal neurogenesis, synaptic plasticity and memory: facts and hypotheses［J］.Rev Neurosci，2007，18（2）：93-114.
［13］Simmons DK，Pang K，Martindale MQ.Lim homeobox genes in the Ctenophore Mnemiopsis leidyi: the evolution of neural cell type specification［J］.Evodevo，2012，3（1）：2-11.
［14］Srivastava M，Larroux C，Lu DR，et al. Early evolution of the LIM homeobox gene family［J］.BMC Biol，2010，8 (1)：4-13.
［15］Zhao Y，Guo YJ，Tomac AC，et al. Isolated cleft palate in mice with a targeted mutation of the LIM homeobox gene lhx8［J］.Proc Natl Acad Sci USA，1999，96（26）：15002-15006.
［16］Manabe T，Tatsumi K，Inoue M，et al. L3/Lhx8 is a pivotal factor for cholinergic differentiation of murine embryonic stem cells［J］.Cell Death Differ，2007，14（6）：1080-1085.