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麻黄素对卵巢组织结构的影响
Effects of ephedrine on ovarian histological structure
目的 探讨麻黄素对雌鼠血液雌二醇（E2）、肾上腺素（EPI）浓度和卵巢组织结构及超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量的影响。 方法 受孕雌鼠分别腹腔注射（2g/L、4g/L、6g/L）麻黄素溶液和等量的生理盐水，采用ELISA法检测15d、20d、25d雌鼠血浆中EPI与E2浓度的变化，用比色法检测卵巢SOD活性及MDA含量的变化，称量检测卵巢的重量变化，用生物显微技术观察卵巢组织结构的变化，统计卵巢各级卵泡的数量。结果 实验组雌鼠血浆中的E2含量降低，EPI含量显著升高，并呈现剂量依赖性，卵巢SOD活性降低，MDA含量显著升高；实验组卵巢的重量明显低于对照组，初级卵泡、次级卵泡和闭锁卵泡数量均增多；黄体数量减少；实验组卵巢固缩，结构不完整，卵母细胞萎缩，颗粒细胞变性、降解，排列疏松、紊乱，黄体结构模糊，颗粒黄体细胞核固缩。 结论 麻黄素影响雌鼠卵巢的组织结构和功能，这可能与卵巢中SOD活性下降有关。
Objective To explore effects of ephedrine on epinephrine （EPI） and estradiol（ E2 ）concentration in blood, histological structures and activity of superoxide dismutase (SOD) and maleic dialdehyde (MDA) content in the ovary of female mice. Methods Pregnant female mice in the experimental group were treated with ephedrine(2g/L，4g/L，6g/L), and the mice in the control group were treated with saline by intraperitoneal injection. Using ELISA method to detect concentration changes of EPI and E2 in plasma, the changes in the enzymatic activity of SOD and MDA content were analyzed by colorimetry. The ovarian weight was measured, histological structures in ovary were observed by microscopy and the number of ovarian follicles were counted. Results EPI concentration in plasma of female mice increased, and the E2 concentration decreased dose-depenently in the experimental group. The activities of SOD were lower, and MDA content significantly increased compared to the control. The ovary weight of experimental groups was lower than the control group. The numbers of primary follicle, secondary follicle and atretic follicle increased and corpus luteum decreased in the experimental groups. There were pyknotic ovarian tissues, including oocyte ateophy, degradation granulose cells, and karyopyknotic granulose lutein cells in the experimental groups. Conclusion Ephedrine has a great effect on the ovarian histological structure and ovarian function of female mice, which may be correlated with the low activities of SOD.
卵巢 / 麻黄素 / 雌二醇 / 超氧化物歧化酶 / 丙二醛 / 组织结构 / 酶联免疫吸附测定 / 雌鼠
Ovary / Ephedrine / Estradiol / Superoxide dismutase / Maleic dialdehyde / Histological structure / ELISA / Female mouse
［1］Wang JJ，Li ChY，Yu ShY，et al.Ephedrine for pups kidney structure and SOD, CAT, and MDA content［J］.Chinese Journal of Zoology，2011，46(6)：118-125.(in Chinese)
王锦锦，李重阳，俞诗源，等.麻黄素对仔鼠肾脏组织结构和SOD、CAT、及MDA含量的影响［J］.动物学杂志，2011，46(6)：118125.
［2］Zhen JW.Pharmacological effects of stimulants and their dependencies Features［M］.Bulletin of Biology，1998，33(9)：2-3.(in Chinese)
郑继旺.中枢兴奋剂的药理作用及其依赖性特点［M］.生物学通报，1998，33(9)：2-3.
［3］Sun J，Li ChY, Yu ShY,et al.Effect on liver tissue structure and related active substances after injecting ephedrine of pups［J］.Acta Anatomica Sinica，2012，43(3)：398-405.(in Chinese)
孙隽，李重阳，俞诗源，等.麻黄素对仔鼠肝脏组织结构和相关活性物质的影响［J］.解剖学报，2012，43(3)：398-405.
［4］Xiao ShN，Yu ShY.Effect on testis structure and Bax, EGF Protein after injecting ephedrine of pups［J］.Chinese Journal of Zoology，2013，48(3)：228-234.(in Chinese)
肖世南，俞诗源.麻黄素对仔鼠睾丸组织结构和Bax、EGF蛋白的影响［J］.动物学杂志，2013，48（3）：228-234.
［5］Li ChY，Gao XJ，Yu ShY，et al. Effect on hippocampus organizational structure and choline acetyltransferase enzyme activity after injecting hroin and ephedrine of pups［J］.Acta Anatomica Sinica，2009，40(6)：826-868.(in Chinese)
李重阳，高先军，俞诗源，等.海洛因、麻黄素对仔鼠下丘脑、海马组织结构及胆碱乙酰基转移酶活性的影响［J］.解剖学报，2009，40(6)：826-868.
［6］Feng HL，Li ChY，Yu ShY，et al.Effects of heroin on SOD and CAT activity and MDA content and histological structure in the lungs of the filial mice and mother mice［J］.Acta Anatomica Sinica，2009，40(2)：288-294.(in Chinese)
冯红丽，李重阳，俞诗源，等.海洛因对仔鼠和母鼠肺中SOD、CAT活力和MDA含量及组织结构的影响［J］.解剖学报，2009，40(2)：288-294.
［7］Wu JM，Li ZJ.G protein-coupled receptors and their signaling pathways ［J］.Progress in Physiological Sciences，2013，44(1)：75-79.(in Chinese)
吴济民，李子健.G蛋白耦联受体及其信号通路［J］.生理科学进展，2013，44(1)：75-79.
［8］Gainetdinov RR ，Premon RT，Bohn LM，et al.Desensitization of G protein-coupled receptors and neuronal function［J］.Annu Rev Neurosci，2004，27：107-144.
［9］Dewire SM，Ahn S，Lefkowitz RJ，et al.Beta-arrestins and cell signaling［J］.Annu Rev Physiol，2007，69：483-501.
［10］Sulzer D，Chen TK，Lau YY，et al.Amphertamine redistributes dopamine from synaptic vesicles to the cytosol and promotes reverse transport［J］. J Neurosci，1995，15(5Pt 2)：4102-4108.
［11］Liu HT，Wang JJ，Zhou YQ.Acte withdrawal in heroin addicts before and after the hypothalamic-pituitary-adrenal axis dysfunction research［J］.Neuroendocrine and Immune，2010，26(10)：907-909.(in Chinese)
刘徽婷，王嘉军，周永芹.海洛因依赖者急性戒断前后下丘脑-垂体-肾上腺轴功能紊乱的研究［J］.神经内分泌与免疫，2010，26(10)：907-909.
［12］Chen BX，Gan XL，Chen Y.Heroin addicts drugs serum IL-1β and IL-4 levels and clinical significance［J］. Journal of Radioimmunology，2005,18(4)：293-294.(in Chinese)
陈伯勋，甘西伦，陈跃.海洛因类药物依赖者戒断后血清IL-1β和IL-4水平及临床意义［J］.放射免疫学杂志，2005，18(4)：293-294.
［13］Fan GW，He J，Wang H，et al.Estrogen and its receptor signal transduction pathway Progress［J］.Chinese Journal of Clinical Pharmacology and Therapeutics，2007，12(3)：266-269.(in Chinese)
樊官伟，何俊，王虹，等.雌激素及其受体信号转导途径的研究进展［J］.中国临床药理学与治疗学，2007，12(3)：266-269.
［14］Guo ShZh，Wu SH，Zhao Y,et al.Effect on gonadal axis and bone tissue after injecting morphine of female rats［J］.Advances in Modern Gynecology，2005，14(3)：210-212.(in Chinese)
郭述真，吴素慧，赵烨，等.吗啡对雌性大鼠性腺轴和骨组织的影响［J］.现代妇科进展，2005，14(3)：210-212.
［15］Boukhliq R，Goodman RLA， Berriman SJ, et al.Subset of gonadotropin-releasing hormone neurons in the ovine medial basal hypothalamus is activated during increased pulsatile luteinizing hormone secretion［J］.Endocrinology，1999，140（12）：5929-5936.
［16］Yin R.Experimental study on the protective effects of curcumin on alcoholic liver injury［D］.Lanzhou:Lan Zhou University，2008.(in Chinese)
尹蓉.姜黄素对酒精性肝损伤保护作用的实验研究［D］.兰州：兰州大学，2008.
［17］Deng HP，Yu ShY，Chen YQ，et al. Effects of X-ray on postnatal body weight, growth of skin and hair, activity and content of superoxide dismutase, catalase, and maleic dialdehyde in liver and kidney of mice［J］.Acta Zoological Sinica，2008，54(6)：1029-1037.(in Chinese)
邓海平，俞诗源，陈玉琴，等.X射线辐射对仔鼠体重、皮毛生长及肝、肾脏组织SOD、CAT活力及MDA含量的影响［J］.动物学报，2008，54（6）：1029-1037.
［18］Zhu H，Ni J，Zhang W，et al.The change of malondialdehyde and superoxide dismutase in apoptotic granulose cell of rat pco［J］.Chinese Journal of Applied Physiology，2001，17(3)：293-295.(in Chinese)
朱辉，倪江，张玮，等.大鼠多囊卵巢颗粒细胞凋亡时MDA含量及SOD活性的变化［J］.中国应用生理学杂志，2001，17（3）：293-295.
［19］Soni MG，Carabin IG，Griffiths JC，et al.Safety of ephedra lessons learned［J］. Toxicol Lett，2004，150(1)：97-110.
［20］Shekelle PG，Hardy ML，Morton SC，et al.Efficacy and safety of ephedrine for weight loss and athletic performance a meta-analysis［J］.JAMA，2003， 289(12)：1537-1545.
［21］Wang X，Huang MSh，Li J，et al.Methamphetamine organism toxicity studies［J］.Huaxi Medical，2003，18 (1)：75-76.(in Chinese)
王雪，黄明生，李静，等. 甲基苯丙胺的机体毒性研究［J］. 华西医学，2003，18(1)：75-76.
［22］Zhen ShP，Lu YX. ATS forensic toxicology research［J］.Chinese Journal of Forensic Medicine, 2004，19(1)：55-57.(in Chinese)
郑少攀，卢延旭.苯丙胺类兴奋剂的法医毒理学研究进展［J］.中国法医学杂志，2004，19（1）：55-57.
［23］Liu DD，Wang B.Studies on female mouse germ cells induced DNA-protein cross-linking used formaldehyde ［J］.Asian Journal of Ecotoxicology，2006，1 (3)：249-253.(in Chinese)刘丹丹，王博.气态甲醛致雌性小鼠生殖细胞DNA-蛋白质交联的研究［J］.生态毒理学报，2006，1 (3）：249-253.
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