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基质细胞衍生因子-1α和白细胞介素-1β诱导淋巴管内皮表型的作用
索宁 王雪颖 杨春林 周辉 李菲 张宗璞 万芳竹 田铧*
解剖学报 ›› 2014, Vol. 45 ›› Issue (3) : 388-392.
基质细胞衍生因子-1α和白细胞介素-1β诱导淋巴管内皮表型的作用
Effect of stromal cell derived factor-1α and interleukin-1β on inducing vascular endothelial cells expressing lymphatic phenotype
目的 探讨基质细胞衍生因子-1α(SDF-1α)及白细胞介素-1β（IL-1β）诱导内皮细胞表达淋巴管表型的作用。方法 SDF-1α和IL-1β分别诱导内皮细胞株CRL-1730，用Real-time PCR、Western blotting及免疫细胞化学等方法检测其内皮及淋巴管标志物，的表达情况。结果 SDF-1α诱导培养之后，CRL-1730细胞株的内皮细胞标志物血管性血友病因子（vWF）、血管内皮钙黏蛋白（VE-cadherin）、血管内皮生长因子受体（VEGFR）2随其浓度增高而表达降低，淋巴管标志物平足蛋白（podoplanin）、同源异形盒蛋白-1（Prox-1）和淋巴管内皮透明质酸受体-1（LYVE-1）随其浓度增高而表达增高。IL-1β诱导之后，CRL-1730细胞株的vWF、VEGFR2和podoplanin、prox-1、LYVE-1的变化趋势同SDF-1α，而VE-cadherin的表达量基本不变。结论 SDF-1α和IL-1β都能够诱导血管内皮细胞表达淋巴管标志物。
Objective To investigate the effect of stromal cell-derived factor-1α (SDF-1α) and interleukin(IL-1β) on inducing vascular endothelial cells to express lymphatic phenotype. Methods The CRL-1730 cell line was cultured and treated with SDF-1α or IL-1β. The expression of endothelial cell markers and lymphatic endothelial cell markers were investigated with Real-time PCR, Western blotting and immunocytochemistry. Results In CRL-1730 cell line, endothelial cell markers such as voln willebrand factor (vWF), VE-cadherin, vascular endothelial growth factor receptor(VEGFR)2, were dose dependently down-regulated after SDF-1α stimulation, while lymphatic phenotypes such as Prox-1, podoplanin and lymphatic vessel endothelial hyaluronan receptor-1(LYVE-1), were dose-dependently up-regulated after SDF-1α stimulation. The changes of vWF, VEGFR2 and podoplanin, Prox-1, LYVE-1 expression after IL-1β stimulation was similar to that after SDF-1α while expression of VE-cadherin changed slightly. Conclusion SDF-1α and IL-1β are able to induce vascular endothelial cell expressing lymphatic phenotype.
基质细胞衍生因子-1α / 白细胞介素-1β / / 同源异形盒蛋白-1 / 血管内皮细胞 / 实时定量聚合酶链反应
Stromal cell-derived factor-1α / Interleukin-1β / Prospero ho-meobox protein-1 / Vascular endothelial cell / Real-time PCR
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山东省自然科学基金;山东省医药卫生科技项目
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