上调分子伴侣mortalin经由MAPK-ERK信号转导通路促进卵巢癌细胞增殖

胡莹莹 韩艳艳 赵佳维 杨玲 刘雯 左伋*

doi:10.3969/j.issn.0529-1356.2014.03.008

解剖学报 ›› 2014, Vol. 45 ›› Issue (3) : 338-343. DOI: 10.3969/j.issn.0529-1356.2014.03.008

肿瘤生物学

上调分子伴侣mortalin经由MAPK-ERK信号转导通路促进卵巢癌细胞增殖

胡莹莹韩艳艳赵佳维杨玲刘雯左伋*

作者信息 +

Mortalin promotes ovarian cancer cell growth through MAPK-ERK signal pathway

HU Ying-ying HAN Yan-yan ZHAO Jia-wei YANG Ling LIU Wen ZUO Ji*

Author information +

文章历史 +

摘要

目的通过获得稳定表达mortalin的卵巢癌细胞株（A2780、A2780/cis），检测mortalin与卵巢癌细胞增殖的关系及可能机制。方法 CCK-8实验检测mortalin过表达组和对照组细胞增殖的变化；通过流式细胞术检测mortalin过表达对卵巢癌细胞周期的影响；Western blotting检测mortalin上调表达后，卵巢癌细胞中MAPK/ERK和JNK/SAPK信号通路蛋白的变化。结果 Mortalin上调表达促进卵巢癌细胞A2780和A2780/cis的增殖。Mortalin通过加快卵巢癌细胞由G₁期快速过渡到G₂/M期,促进细胞增殖，且MAPK/ERK信号通路参与该过程。结论 Mortalin上调表达促进了卵巢癌细胞的增殖，与其对MAPK-ERK信号通路的激活有关。

Abstract

Objective By constructing mortalin stably expressing ovarian cancer cell lines in A2780 and A2780/cis, we demonstrate the role of mortalin in the ovarian cancer cell growth. Methods CCK-8 assay was used to measure cell viability in the overexpression mortalin group compared with the control group. The flow cytometry analysis was used to understand the effect of upregulated mortalin on the ovarian cancer cell cycle. Western blotting was used to determine the expression and phosphorylation level of MAPK/ERK and JNK/SAPK signal pathways. Results The results showed that increased expression of mortalin could accelerate ovarian cancer cell proliferation and promote G₁ transition, leading to a faster restoration of normal distribution of cell cycle. We found that mortalin overexpression significantly activated p-Raf and p-ERK1/2, but not p-JNK. Conclusion The results demonstrate that mortalin effect on the ovarian cancer cell proliferation contributes to active the MAPK-ERK signaling pathway.

导出引用

胡莹莹韩艳艳赵佳维杨玲刘雯左伋*. 上调分子伴侣mortalin经由MAPK-ERK信号转导通路促进卵巢癌细胞增殖[J]. 解剖学报. 2014, 45(3): 338-343 https://doi.org/10.3969/j.issn.0529-1356.2014.03.008

HU Ying-ying HAN Yan-yan ZHAO Jia-wei YANG Ling LIU Wen ZUO Ji*. Mortalin promotes ovarian cancer cell growth through MAPK-ERK signal pathway[J]. Acta Anatomica Sinica. 2014, 45(3): 338-343 https://doi.org/10.3969/j.issn.0529-1356.2014.03.008

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