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黄芪注射液拮抗大鼠脑缺血再灌注损伤的保护作用和机制
Protective effect and mechanism of astragalus injection in cerebral ischemia reperfusion injury in rats
目的 探讨黄芪注射液对大鼠脑缺血再灌注损伤的神经保护作用和机制。方法 成年健康雄性Wistar大鼠70只，应用线栓法经左侧颈外-颈内动脉插线建立大脑中动脉阻塞再灌注模型，经腹腔注射1g/L黄芪注射液（3ml/kg）干预治疗。Longa法评价大鼠神经功能缺损程度，氯化三苯基四氮唑（TTC）染色观察脑梗死体积，苏木素-伊红染色观察顶叶皮质区神经元形态结构变化，透射电子显微镜观察神经细胞的超微结构，流式细胞术检测细胞凋亡，Western blotting检测c-Jun氨基末端激酶3（JNK3）蛋白表达，RT-PCR检测JNK3 mRNA表达。 结果 经黄芪注射液治疗后，大鼠顶叶皮质区神经元JNK3 mRNA和JNK3蛋白表达较对照组显著减低，凋亡细胞数量显著减少，脑梗死体积显著缩小，神经元形态和超微结构以及动物神经行为功能显著改善。结论 黄芪注射液可能通过下调神经元JNK3基因表达而抑制细胞凋亡，缩小脑梗死体积，改善动物的神经行为功能。
Objective To investigate the neuroprotective effect and mechanism of astragalus injection in cerebral ischemia reperfusion injury in rats. Methods A total of 70 healthy adult maleWistar rats were subjected to establish middle cerebral artery occlusion reperfusion models by inserting a monofilament thread from the external-internal carotid artery and treated by injecting 1g/L intraperitoneally astragalus injection (3ml/kg). The neurobehavioral function of rats was evaluated by Longa’s test and the cerebral infarction volume was calculated by tetrazolium chloride staining. The shape and ultrastructure of neurons in parietal cortex were observed by HE stain TEM. The early apoptotic ratio of neurons was detected by flow cytometry. The expressions of c-Jun N-terminal kinase 3 (JNK3) mRNA and protein were determined by RT-PCR and Western blotting, respectively. Results After treatment with astragalus injection, the expressions of JNK3 mRNA and protein reduced significantly, the number of neuronal apoptosis in parietal cortexminus, the cerebral infarction volume shrink, the neuronal shape and ultrastructure and animal neurobehavioral function were improved significantly than those in model group rats. Conclusion The results suggest that astragalus injection may inhibit neuronal apoptosis, reduce the infarction volume and improve the animal neurobehavioral function by down-regulated the expression of JNK3 gene following cerebral ischemia in rats.
脑缺血 / 再灌注损伤 / 黄芪注射液 / c-Jun氨基末端激酶3 / 凋 / 免疫印迹法 / 大鼠
Cerebral ischemia / Reperfusion injury / Astragalus injection / c-Jun N-terminal kinase 3 / Apoptosis / Western blotting / Rat
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国家自然基金
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