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D6S296、D8S264基因座多态性与广西地区汉/壮族人群精神分裂症的关联性
Correlation between D6S296, D8S264 gene polymorphism and schizophrenia of Han/ Zhuang people in Guangxi
目的 探讨D6S296、D8S264基因座多态性与广西地区汉、壮族人群精神分裂症的关联性以及这两个基因座与汉、壮族精神分裂症关联性的差异。
方法 选取广西脑科医院汉族精神分裂症患者及其健康直系家属46对(其中患者46例，家属49例)，壮族精神分裂症患者及其健康直系家属45对（其中患者45例，家属48例）作为研究对象，分别以其健康家属作为正常对照组。提取其全血DNA并使用PCR扩增仪对其进行扩增，所得PCR产物用ABI3730XL型基因分析仪自动进行基因检测，并用遗传学统计方法进行分析。结果 汉、壮族正常对照组D6S296、D8S264各等位基因频率的分布均符合Hardy-Weinberg遗传平衡定律(P＞0.05)。汉、壮族精神分裂症患者组比较，D6S296的264bp等位基因频率分别为2.2％和18.9％（2/17），差异有显著统计学意义(χ 2=13.6，P<0.01)；D8S264的129bp等位基因频率分别为18.5%/8.0%(17/7)，差异有统计学意义(χ 2=4.31，P<0.05)，其他各等位基因频率的分布在各组间的差异均无统计学意义(P＞0.05)。
结论 广西地区汉、壮族人群精神分裂症的发生可能与D6S296、D8S264多态性无关。
Objective To investigate relationship between D6S296, D8S264 gene polymorphism and schizophrenia of Han, Zhuang people in Guangxi as well as its relevant difference. Methods Han patients (46 cases) with schizophrenia and their immediate family members of the health (49 cases), Zhuang patients with schizophrenia (45 cases) and the health (48 cases) were selected as the research object in Brain Hospital of Guangxi,with healthy families as normal control group. To extract the whole blood DNA and use PCR for DNA amplification, the PCR products with ABI3730XL gene analyzer automatically detect, and statistical genetics method were analyzed. Results D6S296/D8S264 of Han, Zhuang normal control group, each allele frequency distribution were accord with Hardy-Weinberg genetic law of balance (P>0.05). The comparison of Han, Zhuang group of patients with schizophrenia, 264 bp of D6S296 allele frequencies were 2.2% and 18.9% (2/17) respectively, with statistically significant difference (χ 2=13.60, P<0.01);129 bp of D8S264 allele frequency were 18.5% / 8.0% (17/7) respectively, and the difference was statistically significant (χ 2=4.31, P<0.05), and distribution of other allele frequency in each group had no statistical significance (P> 0.05). Conclusion Schizophrenia of Han, Zhuang population in Guangxi may have nothing to do with D6S296, D8S264 polymorphism.
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09广西青年基金
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