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Galecin-3在膜联蛋白A7表达抑制的HeLa细胞中的表达变化
Expression of galectin-3 in annexin A7 knockdown HeLa cells
目的 探讨膜联蛋白A7（ANXA7）表达抑制的HeLa细胞galectin-3(gal-3)的表达改变。 方法 将细胞分为实验组、阴性对照组和空白对照组。采用RNA干扰技术将靶向ANXA7的siRNA和阴性对照siRNA脂质体2000转染法分别转染宫颈癌细胞系HeLa细胞，空白对照组不予任何处理。转染48h后采用Western blotting 法和RT-PCR法分别在蛋白水平和mRNA水平进行抑制效果的鉴定。 Western blotting和实时定量PCR法分别检测ANXA7表达抑制的HeLa细胞中galectin-3的表达改变。
结果 靶向ANXA7的siRNA可显著抑制ANXA7的表达；当ANXA7表达受抑后，galectin-3在HeLa细胞中的表达显著下降，与阴性对照组和空白对照组相比，差异有显著性（P<0.05）。 结论 ANXA7表达抑制的宫颈癌细胞系HeLa细胞中galectin-3的表达显著下调，这可能是ANXA7表达受抑的细胞行为学发生改变的机制之一。
Objective To detect the expression of galectin-3（gal-3） in annexin A7 knockdown HeLa cells. Methods The HeLa cells were grouped into an experiment group, a negative group and a blank group. RNA interference technology was used to transfect the siRNA targeted to ANXA7, scrambled siRNA into HeLa cells with lipofectine 2000 without any treatment to the blank group. After transfection for about 48 hours, Western blotting method and PCR method were used to assure the suppression of ANXA7 on both protein level and mRNA level. The expression of galectin-3 was detected with Western blotting and real time qualitative PCR. Results The expression of ANXA7 was significantly suppressed in the cells transfected with ANXA7 siRNA. When ANXA7 expression was suppressed, the expression of galectin-3 decreased significantly compared with the negtive control group and blank control group. The results have significant meaning（P<0.05）. Conclusion The expression of galectin-3 is down-regulated in the ANXA7 suppressed HeLa cells, which may be one of the mechanisms for the abnormal behaviour of the ANXA7 knockdown carcinoma cells.
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