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斑马鱼胚胎发育过程成纤维细胞生长因子受体基因时序性表达
Temporal expressions of fibroblast growth factor receptor genes during Zebrafish embryonic development
目的 探讨斑马鱼胚胎发育过程成纤维细胞生长因子（FGF）受体基因时序性表达规律。 方法 分别提取发育至4、6、10、12、18、24、48及72hpf斑马鱼胚胎总RNA并反转录为cDNA，用实时定量PCR方法检测FGF受体基因（fgfrs）mRNA的相对表达量。 结果 斑马鱼胚胎发育过程fgfrs mRNA动态表达水平有明显峰谷变化（P<0.01），胚胎fgfrs开始表达阶段依次为fgfr1a与fgfr1b（囊胚期）、fgfr4（原肠胚初期）、fgfr2（原肠胚期）及fgfr3（原肠胚后期），其表达高峰期分别在原肠胚期、原肠胚后期、体节期及胚胎发育中后期。旁系同源基因fgfr1a与fgfr1b动态表达趋势相似（r=0.830, P<0.05）。fgfr1a与fgfr3、fgfr1b与fgfr4、fgfr2与fgfr3 mRNA表达量之间相关分析也有意义（r值分别为 -0.726, 0.821及0.772,P<0.05）。 结论 斑马鱼胚胎发育过程fgfrs时序性表达有一定规律性，fgfr1、fgfr4主要参与胚胎早期发育调控，fgfr2、fgfr3主要参与胚胎中、后期发育调控。fgfr1a与fgfr1b共表达使其调控作用具有冗余能力。fgfr1和fgfr3之间在表达上可能存在相互制约关系。
Objective To study the temporal expressions of fibroblast growth factor (FGF) receptor genes (fgfrs) during zebrafish embryonic development. Methods Total RNA was extracted from zebrafish embryos at 4, 6, 10, 12, 18, 24, 48 and 72 hours post fertilization (hpf), respectively, and was reversely transcripted to cDNA. The fgfrs’ mRNA levels were then determined by real time quantitative polymerase chain reaction (Real-time PCR). Results The temporal mRNA expression of fgfrs varied significantly during embryonic development with a pattern of peak curve (P<0.01). During zebrafish embryogenesis the mRNA expressions of fgfr1a-b, fgfr4, fgfr2 and fgfr3 began at blastula, early gastrula, gastrula and late gastrula, and peaked at gastrula, late gastrula, segmentation and mid-late period, respectively. There was significant correlation between the expression levels of the paralogs fgfr1a and fgfr1b (r=0.830,P<0.05). And there were also significant correlations in fgfr1a-fgfr3, fgfr1b-fgfr4 and fgfr2-fgfr3 (P<0.05) with r values of -0.726, 0.821 and 0.772, respectively. Conclusion Each of fgfrs has its own expression pattern during zebrafish embryonic development. The fgfr1 and fgfr4 mainly play their roles at the early period of embryogenesis, while the fgfr2 and fgfr3 at the mid-late period. The co-expression of fgfr1a and fgfr1b exhibits the redundant function of the paralogs during embryonic development. There probably is a reciprocal relationship in gene expression between fgfr1 and fgfr3, But the intrinsic correlations of fgfr1-fgfr4 and fgfr2-fgfr3 still need to be verified.
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国家自然科学基金
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