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小鼠海马发育中Bcl-2和Bax表达的体视学观察
Stereoscopic study on the expression of Bcl-2 and Bax in development of mice hippocampus
目的 探讨B淋巴细胞瘤-2基因（Bcl-2）和促凋亡基因Bax(Bax）在C57/BL6小鼠海马发育过程中的表达变化。 方法 取胚龄(E) 18、20 d和生后(P)1、3、7、14、21、28 d以及2、3、6、15、18个月的C57/BL6小鼠海马，每组8只，应用免疫组织化学技术及体视学方法检测Bcl-2和Bax 蛋白的表达。结果 E18 d～P21 d，在齿状回（DG）的颗粒层、海马阿蒙角（CA）以及CA各区（CA1～CA4）的锥体层，Bcl-2和Bax 阳性细胞及其体密度均呈现出先逐渐增加再逐渐降低的趋势，除DG区Bax阳性细胞及其体密度在P14 d达到最高外，其他均在P7 d达到最高（P<0.01）。P28 d后均趋于稳定（P>0.05）。 CA锥体层及DG 颗粒层Bcl-2/Bax的体密度比值在P1d显著降低（P<0.01），之后趋于稳定（P>0.05）。 结论 小鼠海马Bcl-2和Bax的表达在胚胎发育晚期和生后发育早期较多，而在成年及老年期的表达稳定在较低水平，它们可能参与了海马的塑形过程。
Objective To observe the expression of Bcl-2 and Bax in the development of C57/BL6 mice hippocampus. Methods Mice hippocampus of Embryos (E) 18 day (d), E 20 d, postnatal (P) 1 d, P 3 d, P 7 d, P 14 d, P 28 d, P 2 month (M), P 3 M, P 6 M, P 15 M, P 18 M were used in this study. Immunohistochemistry and stereoscopy were used to detect the expression of Bcl-2 and Bax protein in the mice hippocampus at different ages. Results From E18 d to P21 d, Bcl-2 and Bax immunoreactive cells in both Ammon’cornu (CA) and dentate gyrus (DG) firstly and gradually increased and then decreased（P<0.01）. The volume density of both Bcl-2 and Bax immunoreactive cells came to the top on P7 d except the Bax expression in DG which was the highest on P14 d（P<0.01）.
Conclusion The expression of Bcl-2 and Bax is high at the early stage of hippocampus development and then low at its adult stage and senectitude, which may be involved with the forming of hippocampus.
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