欢迎访问《解剖学报》官方网站！今天是
English
促性腺激素释放激素受体在雌性山羊颈胸神经节中的分布
Distribution of gonadotropin releasing hormone receptor in cervicothoracic ganglion of female goats
目的 探讨颈胸神经节是否具备接受促性腺激素释放激素（GnRH）作用的条件。方法 实验采用免疫组织化学SP法，观察GnRH受体在雌性山羊颈胸神经节中的分布特点。结果 在颈胸神经节中，神经元胞体均为GnRH受体免疫反应阳性，其细胞膜以及膜下胞质中的颗粒状物质呈棕黄色，GnRH受体为强阳性表达，而胞核呈阴性表达。部分神经元可见其突起，呈黄色，GnRH受体为中等阳性表达。卫星细胞也呈黄色，GnRH受体为中等阳性表达。图像分析表明，呈阳性反应的神经元胞体与节内其他阳性成分相比，其GnRH受体相对表达量差异性极显著（P＜0.01）。结论 在雌性山羊颈胸神经节中，神经元胞体是GnRH发挥作用的主要靶细胞，颈胸神经节具备接受GnRH作用的条件。提示GnRH有可能通过作用于颈胸神经节交感节后神经元上的GnRH受体来影响心肺器官的功能活动，即颈胸神经节可能是心肺功能活动中协调GnRH内分泌调节和该神经节自主神经调节两途径之间的中继站。
Objective To explore whether cervicothoracic ganglion has the condition by which gonadotropin releasing hormon(GnRH) can exert its function. Methods Immunohistochemical SP method was adopted to observe the distribution of GnRH receptor (GnRHR) in cervicothoracic ganglion of female goats. Results The results showed that, all the neurons in cervicothoracic ganglion responded positively to GnRHR. The membrane of neurons and the granular material in cytoplasm were strong positive. The neuronal nucleus was negative to GnRHR while neurites and satellite cells showed medium positive. Image analysis showed a significant difference of GnRHR expression between neurons and non-neurons (P＜0.01).Conclusion The results indicate that neurons in cervicothoracic ganglion are the main target cells of GnRH, and cervicothoracic ganglion has the condition by which GnRH can exert its function, which implies GnRH may regulate the function of heart and lung through the GnRHR distributed in cervicothoracic ganglion. Thus，the GnRHR existing in these neurons may be a “transfer station” for GnRH to coordinate heart and lung activity through endocrine and autonomic nerve pattern.
［1］Stojilkovi SS， Reinhart J, Catt KJ. Gonadotropin-releasinghormone receptors: structureandsignal transduction prthways［J］. Endocr Rev, 1994, 15(4): 462-499.
［2］Troskie B, King JA, Millar RP, et al. Chicken GnRHⅡ-like peptides and a GnRH receptor selective for chicken GnRHⅡ in amphibian sympathetic ganglia［J］. J Neuroendocrinology, 1997, 65(6): 396-402.
［3］Fan J, Ming J, Chen WD, et al. Distribution characteristics of GnRh receptor in the middle cervical ganglion of goat［J］. Chinees Veterinary Science, 2012, 42(7): 737-741.(in Chinese)
范洁，明佳，陈文东，等. GnRH受体在山羊颈中神经节的分布特点［J］. 中国兽医科学，2012，42(7)：737-741.
［4］Dong F, Skinner DC, Wu TJ, et al. The heart: novel gonadotrophin-releasing hormone target［J］. J Neuroendocrinol, 2011, 23(5): 456-463.
［5］Wang YH, Tian XE, Jiang ZhL, et al. Application of GnRH and its analogue in animal breeding［J］. Chinees Journal of Animal Science, 2013, 49(3): 89-92.(in Chinese)
王一涵，田秀娥，江中良，等. GnRH及其类似物在动物繁殖中的应用概述［J］. 中国畜牧杂志，2013，49(3)：89-92.
［6］Arora KK, Sakai A, Catt KJ. Effects of second intracellular loop mutations on signal transduction and internalization of the gonadotropin-releasing hormone receptor［J］. J Biol Chem, 1995, 270(29): 22820-22826.
［7］Gault PM, Morgan K, Pawson AJ, et al. Sheep exhibit novel variations in the organization of the mammalian typeⅡ gonadotropin-releasing hormone receptor gene［J］. Endocrinology, 2004, 145(5): 2362-2374.
［8］Skinner DC, Albertson AJ, Navratil A, et al. Effects of gonadotropin-releasing hormone outside the hypothalamic-pituitary-reproductive axis［J］. J Neuroendocrinol, 2009, 21(4): 282-292.
［9］Albertson AJ, Navratil A, Mignot M, et al. Immunoreactive GnRH typeⅠreceptors in the mouse and sheep brain［J］. J Chem Neuroanat, 2008, 35(4): 326-333.
［10］Iwakoshi E, Takuwa K, Fujisawa Y, et al. Isolation and characterization of a GnRH-like pepfide from octopus vulgaris［J］. Biochem Biophys Res Commun, 2002, 291(5): 1187-1193.
［11］Wang BH, Liang ZhH, Sun HM. Comparative effects of the two methods of stellate ganglion block on the recurrent laryngeal nerve［J］. Pain Clinic Journal, 2010, 6(6): 430-432.(in Chinese)
王宝宏，粱志宏，孙慧民. 两种星状神经节阻滞方法对喉返神经影响的对比观察［J］. 实用疼痛学杂志，2010，6(6)：430-432.
［12］Zhao SZh, Zhang L, Meng FM, et al. Effects ofleft stellate ganglion block on in patients undergoing coronary artery bypass grafting［J］. Chinese Journal of Anesthesiology, 2009, 2(29): 105-107.(in Chinese)
赵素贞，张立，盂凡民，等. 左侧星状神经节阻滞对冠状动脉旁路移植术患者心功能的影响. 中华麻醉学杂志，2009，2(29)：105-107.
［13］Zhang QX, Zeng ShH, Zeng R, et al. Clinical research of stellate ganglion block for unstable angina［J］. Chinese Journal of Trauma and Disability Medicine, 2007, 15(4): 18-19.(in Chinese)
张清祥，曾胜宏，曾荣，等. 星状神经节阻滞治疗不稳定型心绞痛的临床研究［J］. 中国伤残医学，2007，15(4)：18-19.
［14］Yang CZh. The affect of right stellate ganglion block on circular reaction of endotracheal intubation under general anesthesia［J］. The Journal of Medical Theory and Practice, 2002, 15(11): 1258-1259.(in Chinese)
杨慈洲. 单侧星状神经节阻滞对全麻气管插管循环反应的影响［J］. 医学理论与实践，2002，15(11)：1258-1259.
［15］Quintanar JL, Salinas E. Neurotrophic effects of GnRH on neurite outgrowth and neurofilaament protein expression in cultured cerebral cortical neurons of rat embryos［J］. Neurochem Res, 2008, 33(6): 1051-1056.
［16］Yang SN, Lu F, Wu JN, et al. Activation of gonadotropin-releasing hormone receptors induces along-term enhancement of excitatory postsynaptic currents mediated by ionotropic glutamate receptors in the rat hippocampus［J］. Neurosci Lett, 1999, 260(1): 33-36.
［17］Kawai T, Abe H, Akazome Y, et al. Neuromodulatory effect of GnRH on the synaptic transmission of the olfactory bulbar neural circuit in goldfish, carassius auratus［J］. J Neurophysiol, 2010, 104(6): 3540-3550.
国家自然科学基金项目;陕西省自然科学基金项目
/
| 〈 |
|
〉 |
