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桉叶油对维甲酸干扰大鼠胚胎植入及生长发育的影响
Effect of eucalyptus oil on rat embryo implantation and growth interfered with retinoic acid
目的 探讨桉叶油对维甲酸干扰大鼠胚胎植入及生长发育的影响。方法 SD孕鼠42只，随机分为6组,FA组正常对照组、溶剂组(花生油+RA)、3个实验组（桉叶油高、中、低剂量+RA）及RA组。溶剂组用花生油2ml/只于孕第7~14天灌胃,每天1次，孕第10天维甲酸40mg/kg灌胃1次。桉叶油3个剂量组分别用300mg/kg、200mg/kg、100mg/kg的桉叶油于孕第7~14天灌胃,每天1次，孕第10天维甲酸40mg/kg灌胃1次。RA组用40mg/kg的维甲酸于孕第10天灌胃1次。正常对照组给予自由摄食饮水。各组于孕21d早上处死孕鼠，取胚胎，记录孕鼠体重，子宫重，胎盘重。计胚胎植入总数，吸收胎数、活胎数、死胎数。观察胚胎外形，并测量胎鼠体重、身长、尾长。结果 正常对照组、桉叶油+RA和溶剂+RA组孕鼠增重、子宫重、胎盘重与RA组比较均无差异；溶剂+RA组的孕鼠的胎盘重较其他各组低，与RA和桉叶油+RA各组比较差异无显著性，与正常对照组比较有差异（P<0.05）。正常对照组的活胎率 ［（97.3±4.6）%］较灌胃维甲酸各组的活胎率高（P<0.05），吸收胎率［（2.6±4.6）%］和畸形率（0）较灌胃维甲酸各组低（P<0.05），死胎率为0；在灌胃维甲酸各组中，桉叶油高［（79.6±14.5）%］、中［（67.6±30.8）%］剂量组的活胎率较RA［（58.6±26.6）%］组高，但比较无差异；畸胎率［（44.5±41.6)%；(57.5±35.1）%］较RA［（68.1±43.6）%］组低，但差异无统计学意义。灌胃维甲酸各组的吸收胎率比较无差异。正常对照组的胎鼠体重、身长、尾长均值明显高于灌胃维甲酸各组胎鼠的体重、身长、尾长均值 (P<0.05);在灌胃维甲酸各组中，桉叶油各组胎鼠体重、身长的均值明显高于RA组和溶剂对照组 (P<0.05)；溶剂对照组胎鼠体重、身长、尾长与RA组比较无差异；桉叶油各组胎鼠尾长的均值均高于RA组，但比较无差异。 结论 维甲酸干扰了大鼠胚胎的植入和发育，桉叶油对RA干扰大鼠胚胎植入有一定的拮抗作用，对RA引起的胎鼠生长发育迟缓有一定的抑制作用。
Objective To study the effect of eucalyptus oil on retinoic acid(RA) interference in rat embryo implantation and growth. Methods Forty-two pregnant SD rats were randomly divided into 6 groups,7 rats each: 3 experiment groups ( high, medium, low doses of Eucalyptus+RA), a solvent group (peanut oil +RA), a retinoic acid group (RA) and a normal group with normal diet. Three experiment groups and Solvent group were lavaged from gestation 7th to 14th day with eucalyptus oil in 300 mg/kg, 200 mg/kg, and 100 mg/kg and peanut oil 2ml per animal per day, respectively. Each of them was garaged with retinoic acid (40mg/kg) at gestation 10th day. RA group was garaged with 40 mg/kg at gestation 10th day. All pregnant rats were sacrificed at 21st day. The weights of the body, uterus and placenta of the pregnant rats were measured. The number of absorbed, live and dead fetus were counted respectively. The length of fetus body and tail as well as body weight were measured. Results The uterus and placenta weight, change in body weight of pregnant rats in normal group, Eucalyptus oil groups and Solvent group showed no significant difference compared with RA group. The solvent group rats’ placental weight was lower than that in eucalyptus oil groups’ and RA group’s (P>0.05), but it was significantly lower than that in normal group (P<0.05). The rate of live fetus in normal group was more than that in other groups with significant difference (P<0.05). The teratogenic rate and the absorption rate of normal group were lower than that of the other groups’ (P<0.05). The mortality of normal group was zero. In gavaged retinoic acid groups, the rates of live fetus in high, medium of eucalyptus oil group were higher than that in RA group (P>0.05). It’s teratogenic rate was lower than that of RA group(P>0.05). The absorption rate in each eucalyptus oil group and solvent group compared with RA group had no difference. The fetus body weight, body length, and tail length of Normal group were significantly higher than that in the other groups（P<0.05). In gavaged retinoic acid groups, the fetus body weight and body length of each eucalyptus oil group were significantly higher than that in solvent group and RA group（P<0.05). The fetus body weight, body length, tail length of solvent group had no significant difference compared with the RA group (P>0.05). The fetus tail length in eucalyptus oil groups was higher than that in RA group, but no significant differences were found(P>0.05). Conclusion Retinoic acid interferes the rat embryo implantation and development; Eucalyptus oil has certain influence on the RA interference of rat embryo implantation, and it also has certain inhibition on fetal growth retardation.
桉叶油 / 维甲酸 / 致畸 / 胚胎发育 / 生殖毒性实验 / 大鼠
Eucalyptus oil / Retimoic acid / Teratogenicity / Embryonic development / Reproductive toxicity testing / Rat
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