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ROBO4在上皮性卵巢癌组织和血清中的表达及临床意义
Expressions and clinical significance of ROBO4 in both serum and tumor tissues in patients with epithelial ovarian cancer
目的 探讨ROBO4在上皮性卵巢癌组织和血清中的表达及其在卵巢癌发生发展中的作用。方法 采用免疫组织化学和反转录聚合酶链反应（RT-PCR）方法检测110例卵巢癌，54例卵巢良性肿瘤，40例正常卵巢组织中ROBO4蛋白和ROBO4mRNA表达，比较其表达与卵巢癌临床病理特征的关系；并采用ELISA检测卵巢癌、卵巢良性肿瘤和正常人血清中ROBO4表达水平。结果 卵巢癌组织中ROBO4蛋白和ROBO4mRNA表达显著低于正常对照组和卵巢良性肿瘤组（P<0.05）；上皮性卵巢癌血清中ROBO4的表达明显低于卵巢良性肿瘤和正常人（P<0.05）；卵巢癌组织中ROBO4表达降低与卵巢癌FIGO分期、淋巴结转移、大网膜转移和腹水相关（P<0.05）；卵巢癌组织中ROBO4表达与年龄、组织学类型和病理分级无相关性。卵巢癌患者血清ROBO4、CA125的相关分析显示，两者之间无相关性。结论 在卵巢癌发生过程中ROBO4表达降低起到重要作用，表达异常的ROBO4与卵巢癌的侵袭转移能力相关，并与卵巢癌的临床病理学特征有明显的相关性。
Objective To explore the levels of ROBO4 and its roles in patients with ovarian tumor, and to investigate the correlation between the expressions of ROBO4 in ovarian tumor tissues and the related clinicopathological characteristics. Methods We examined expression of ROBO4 in 40 cases of the normal ovarian tissue, 54 cases of the ovarian cyst tissue, and 110 cases of ovarian cancer by using the immunohistochemistry and RT-PCR, and compared with clinicopathological features of ovarian cancer. Serums were checked by ELISA in patients with ovarian cancer and ovarian benign tumors and health women.
Results The intensities of ROBO4 protein immunohistochemical positive staining and its mRNA expression were significantly lower in ovarian cancer than those in both normal ovarian tissues and benign tumor tissues (P<0.05). The serum ROBO4 in patients with malignant ovarian tumor was significantly lower than those in healthy women and benign tumors (P<0.05). Loss or reduced expression of ROBO4 was related with clinical stages, lymph node metastasis or omental metastasis or ascites of ovarian cancer (P<0.05), but not related with age, pathologic types, and histological grade. The correlation analysis showed that there was no correlation between serum ROBO4 and CA125. Conclusion Reduced expression of ROBO4 plays an important role in tumorigenesis of ovarian cancer, which is related to invasion and metastasis of ovarian cancer.
卵巢癌 / ROBO4 / 免疫组织化学 / 反转录聚合酶链反应 / 人
Ovarian carcinomas / ROBO4 / Immunohistochemistry / RT-PCR / Human
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国家高技术研究发展计划（863计划）卵巢癌分子分型和个体化诊疗技术;特异性扩增活化自体淋巴细胞（EAAL）治疗卵巢癌的作用机制
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