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人早孕绒毛细胞滋养层细胞培养方法的改良
Isolation and culture of first trimester human trophoblast cell
目的 探索一种简便高纯度的早孕滋养细胞的体外培养方法。方法 采用完整早孕绒毛通过胰蛋白酶联合胶原酶消化法分离，Percoll梯度分离法提纯体外培养，应用光镜HE染色和免疫细胞化学法检测细胞纯度。 结果 CK-7表达阳性细胞数可以达到90%以上。结论 完整绒毛消化和Percoll纯化联合可在短期内获得高纯度、高产量的滋养细胞以供后期试验，方法更加简便。
Objective To research a simple method for purification of first trimester human trophoblast cell in vitro.Methods Whole first trimester human chorionic tissues were isolated with trypsin/collagenase. HE staining and immunocytochemistry methods were used to detect the tyophoblast cells purity. Results Cytokeratin-7 positive cells were more than 90%. Conclusion First trimester human Trophoblast cells can be obtained through the simple method and may offer enough cells for later experiments.
滋养细胞 / 早孕 / 原代细胞培养 / Percoll分离 / 人
Tyophoblast cell / First trimester / Primary cell culture / Percoll gradient / Human
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吴迪，贾永峰，吴岩.细胞永生化与细胞癌变之间关系的研究进展［J］. 中国组织工程研究与临床康复, 2010,14(45):8491-8494.
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