欢迎访问《解剖学报》官方网站！今天是
English
小鼠胚胎呼吸内胚层形态发生与咽前间充质发育及流出道分隔的关系
李慧超 景雅* 师亮 杨艳萍 李海荣 乔从进 乔爱秀 蔡玉瑾
解剖学报 ›› 2013, Vol. 44 ›› Issue (6) : 804-811.
小鼠胚胎呼吸内胚层形态发生与咽前间充质发育及流出道分隔的关系
Association of morphogenesis of pulmonary endoderm with development of prepharyngeal mesenchyme and outflow tract septation in mouse embryos
目的 探讨呼吸内胚层与咽前间充质细胞发育的关系及对小鼠胚胎心流出道分隔的影响。方法 45只胚龄8~13d小鼠胚胎心连续石蜡切片，用抗胰岛因子1（ISL-1）、抗α-平滑肌肌动蛋白（α-SMA）、抗音猬因子（音速波状蛋白， Shh）、抗patched（Ptc1）、抗patched 2（Ptc2）、抗smoothened（Smo）及抗心肌肌球蛋白重链（MHC）抗体进行免疫组织化学及免疫荧光染色。 结果 胚龄8～9d，ISL-1阳性细胞分布在心包腔背侧壁及前肠两侧间充质，并延伸至原始心管动脉端，心管心肌显较强的Ptc1和Ptc2阳性表达。胚龄10~13d，呼吸内胚层向腹侧延伸，Ptc1和Ptc2呈较强表达，ISL-1阳性咽前间充质细胞围绕呼吸内胚层形成对称的特征性锥体形结构，经动脉囊背侧壁伸入动脉囊腔，形成主肺动脉隔。胚龄12d，主肺动脉隔ISL-1阳性表达基本消失，大部分细胞转变为α-SMA阳性细胞。 结论 呼吸内胚层的分化发育与咽前ISL-1阳性间充质细胞的发育聚集密切耦联。发育中的呼吸内胚层可能作为组织中心，通过Shh信号通路对ISL-1阳性细胞的聚集提供位置信息。呼吸内胚层的正常腹侧延伸不但可诱导ISL1阳性细胞的正常迁移和流出道的正常分隔，对流出道的正常形态发生及有效的肺循环建立起重要作用。
Objective To explore the relationship of pulmonary endoderm with the development of prepharyngeal mesenchyme and the outflow tract septation of the mouse embryonic heart. Methods Both the immunohistochemical and immunofluorescent methods were used in the present studies in order to observe islet-1(ISL-1), α-smooth muscle actin(α-SMA), sonic hedgehog(Shh); patched(Ptc1), patched 2(Ptc2), smoothened(Smo) and myosin heavy chain(MHC) distribution in 45 mice embryos from embryonic day(ED)8 to embryonic day(ED)13. Results From ED8 to ED9, ISL-1 positive cells distributed in the splanchnic mesoderm of the pericardial cavity dorsal wall, bilateral mesenchyme to the foregut, and extended distally to the primary heart tube. Ptc1, Ptc2 positive cells were detected in the myocardium of the heart tube. During ED10 to ED13, developing pulmonary endoderm extended ventrally and expressed Ptc1, Ptc2 strongly. ISL-1 positive prepharyngeal mesenchyme surrounding the pulmonary endoderm formed a symmetrically distinctive cone-shaped structure, which projected into the aortic sac to form the primary aorta-pulmonary septum. At ED12, most of the cells in aorta-pulmonary septum turned to express α-SMA, only a few ISL-1 positive cells could be detected. Conclusion The differentiation and development of pulmonary endoderm are closely associated with typical aggregation of ISL-1 positive prepharyngeal mesenchyme. The developing pulmonary endoderm probably acts as an organizing center via sonic hedgehog signaling pathway to confer positional information on ISL-1 positive cells aggregation. The ventral extension of the pulmonary endoderm may not only induce the accumulation and migration of ISL-1 positive cells, but also play important roles in normal morphogenesis of the outflow tract and establishment of effective pulmonary circulation.
呼吸内胚层 / 胰岛因子1阳性细胞 / 心流出道 / 音猬因子 / 免疫组织化学 / 免疫荧光 / 小鼠
Pulmonary endoderm / Islet-1 positive cell / Outflow tract of the heart / Sonic hedgehog / Immunohistochemisty / Immunofluorescence / Mouse
［1］Cohen MM Jr. Hedgehog signaling update［J］. Am J Med Genet A, 2010, 152A(8):1875-1914.
［2］Lin LZ, Bu L, Cai CL, et al. Isl1 is upstream of sonic hedgehog in a pathway required for cardiac morphogenesis［J］. Dev Biol, 2006, 295(2):756-763.
［3］Goddeeris MM, Schwartz R, Klingensmith J, et al. Independent requirements for Hedgehog signaling by both the anterior heart field and neural crest cells for outflow tract development［J］. Development, 2007, 134(8):1593-1604.
［4］Hoffmann AD, Peterson MA, Friedland-Little JM, et al. Sonic hedgehog is required in pulmonary endoderm for atrial septation［J］. Development, 2009, 136(10):1761-1770.
［5］Sun Y, Liang X, Najafi N, et al. Islet1 is expressed in distinct cardiovascular lineages, including pacemaker and coronary vascular cells［J］. Dev Biol, 2007, 304(1):286-296.
［6］Cai CL, Liang X, Shi Y, et al. Isl1 identifies a cardiac progenitor population that proliferates prior to differentiation and contributes a majority of cells to the heart［J］. Dev Cell, 2003, 5(6):877-889.
［7］Wu ShSh, Jing Y, Yang YP, et al. Association of neural crest cells and ISL-1 positive cells with the development of the outflow tract of the mouse embryonic heart［J］. Acta Anatomica Sinica, 2011, 42(5):684-689.(in Chinese)
武姗姗，景雅，杨艳萍，等. 神经嵴细胞和胰岛因子1阳性细胞与小鼠胚胎心流出道发育的关系［J］. 解剖学报，2011，42（5）：684-689.
［8］Wang JJ, Zhao RQ, Li HR, et al. GATA4 expression during primary heart tube formation and venous pole development of the mouse embryonic heart ［J］. Acta Anatomica Sinica, 2009, 32(4):437-439. (in Chinese)
王晶晶，赵瑞庆，李海荣，等. 转录因子GATA4与原始心管的形成和静脉端的发育［J］. 解剖学报，2009，32（4）：437-439.
［9］Rochais F, Mesbah K, Kelly RG. Signaling pathways controlling second heart field development［J］. Circ Res, 2009, 104(8):933-942.
［10］Dyer LA, Kirby Ml. Sonic hedgehog maintains proliferation in secondary heart field progenitors and is required for normal arterial pole formation［J］. Dev Biol, 2009, 330(2):305-317.
［11］Dessaud E, McMahon AP, Briscoe J. Pattern formation in the vertebrate neural tube: A sonic hedgehog morphogen-regulated transcriptional network［J］. Development, 2008, 135(15): 2489-2503.
［12］Johnson RL, Tabin C. The long and short of hedgehog signaling［J］. Cell, 1995, 81(3):313-316.
［13］Incardona JP, Gruenberg J, Roelink H. Sonic hedgehog induces the segregation of patched and smoothened in endosomes［J］. Curr Biol, 2002, 12(12):983-995.
［14］Katoh Y, Katoh M. Hedgehog signaling, epithelial-to-mesenchymal transition and miRNA［J］. Int J Mol Med, 2008, 22(3):271-275.
［15］Ghatpande S, Ghatpande A, Zile M, et al. Anterior endoderm is sufficient to rescue foregut apoptosis and heart tube morphogenesis in an embryo lacking retinoic acid［J］. Dev Biol, 2000, 219(1):59-70.
［16］Moore KL, Persaud TVN. The Developing Human: Clinically Oriented Embryology［M］. 8th, ed. China:Saunders, 2008:314.
［17］Li Y, Zhang H, Choi SC, et al. Sonic hedgehog signaling regulates Gli3 processing, mesenchymal proliferation, and differentiation during mouse lung organogenesis［J］. Dev Biol, 2004, 270(1):214-231.
［18］Cai YJ, Jing Y, Qiao AX, et al. Coupling of heart ourtflow tract morphogenesis with development of pulmonary endoderm in mouse embryo［J］. Acta Anatomica Sinica, 2012, 43(6):802-806. (in Chinese)
蔡玉瑾，景雅，乔爱秀，等. 小鼠胚胎心流出道的形态发生与呼吸内胚层发育耦联［J］. 解剖学报，2012，43（6）：802-806.
山西医科大学大学生创新基金资助项目;省自然科学基金资助项目;其他(不属于以上基金类别的请自行输入下框);国家自然科学基金资助项目;国家留学基金委资助项目
/
| 〈 |
|
〉 |
