欢迎访问《解剖学报》官方网站！今天是
English
胰岛淀粉样多肽、葡萄糖转运蛋白2在海洛因戒断、脱毒和复吸大鼠胰岛β细胞中的表达
韩晶 梁文妹* 洪艳 夏白娟 胡赟 李一欣
解剖学报 ›› 2013, Vol. 44 ›› Issue (6) : 795-799.
胰岛淀粉样多肽、葡萄糖转运蛋白2在海洛因戒断、脱毒和复吸大鼠胰岛β细胞中的表达
Expression of islet amyloid polypeptide and glucose transporter 2 in the islet β cell of rat during heroin withdrawal, detoxification and relapse
Objective To investigate the expression changes of islet amyloid polypeptiole(IAPP) and glucose transporter 2(GLUT2) in islet β cells during heroin withdrawal induced by naloxone, methadone detoxification treatment, and heroin relapse. Methods Male SD rats (n=32) were randomly divided into a normal control group (NCG, n=8) and an experiment group (EG, n=24). The EG was further divided into heroin withdrawal group (HWG), methadone detoxication group (MDG), and heroin relapse group (HRG). Pancreas was drawn from each group. Immunohistochemistry and image analysis were used to detect the expression of IAPP and GLUT2. Results As compared with the normal control, the immunostainning of IAPP and GLUT2 was stronger in β cells of islets from heroin withdrawal and relapse rats than that in controls; the numerical density on area of IAPP also increased; the mean gray values decreased in these two groups. No difference was found in the immunostainning, the mean gray values and the numerical density on area during detoxification treatment compared to controls. Conclusion During heroin withdrawal and relapse, the synthesis of IAPP and GLUT2 increased, while they became decreased in methadone detoxification. The changes of IAPP and GLUT2 were consistent and had same rhythm in three stages. IAPP and GLUT2 took part in energy metabolic process, which may be related to food intake and body weight, or protective function toβcells.
objects To investigate the expression changes of IAPP and GLUT2 in islet βcells during heroin withdrawal induced by naloxone, methadone detoxification treatment, and heroin relapse. Methods Male Sprague-Dawley rats were divided into normal control group (NCG) and experiment group (EG). The EG was further divided into heroin withdrawal group (HWG), methadone detoxication group (MDG), and heroin relapse group (HRG). Pancreas was drawn from each group. Immunohistochemistry and image analysis were used to detect the expression of IAPP and GLUT2. Results As compared with the normal control, the immunostainning of IAPP and GLUT2 turned express intensive inβcells of islets from heroin withdrawal and relapse rats than that in controls; the numerical density on area of IAPP also increased; the mean gray values decreased in these two groups. There is no difference were found in the immunostainning, the mean gray values and the numerical density on area during detoxification treatment compared to controls. Conclusion During heroin withdrawal and relapse, the expression increased. While in detoxification, they became decreased. The changes of IAPP and GLUT2 are consistent and had same rhythm in three stages. It showed that IAPP and GLUT2 take participate in energy metabolic process, , which may be related to food intake and body weight, or protective function toβcells.
胰岛淀粉样多肽 / 葡萄糖转运蛋白2 / 胰岛β细胞 / 海洛因 / 美沙酮 / 复吸 / 免疫组织化学 / 大鼠
Islet amyloid polypeptide / Glucose transporter 2 / Islet &beta / cell / Heroin / Methadone / Relapse / Immunohistochemistry / Rat
［1］Naujok O, Francini F, Picton S, et al. Changes in gene expression and morphology of mouse embryonic stem cells on differentiation into insulin-producing cells in vitro and in vivo［J］.Diabetes Metab Res Rev, 2009, 25(5):464-476.
［2］Min DK, Tuor UI, Koopmans HS, et al. Changes in differential functional magnetic resonance signals in the rodent brain elicited by mixednutrient or protein-enriched meals ［J］. Gastroenterology, 2011,141(5):1832-1841.
［3］Beall C, Piipari K, Al-Qassab H, et al. Loss of AMP-activated protein kinase alpha2 subunit in mouse beta-cells impairs glucose-stimulated insulinsecretion and inhibits their sensitivity to hypoglycaemia［J］.Biochem J, 2010, 429(2):323-333.
［4］Liang WM, Liu X, Li ZhCh, et al. The effects of heroin dependence on expression of islet amyloid polypeptide and glucose transporter 2 in pancreas islet β cells of rats ［J］. Acta Anatomica Sinica, 2009, 40(1):108-112. (in Chinese)
梁文妹, 刘霞, 李占淳, 等. 海洛因依赖对大鼠胰岛β细胞表达胰岛淀粉样多肽和葡萄糖转运蛋白2的影响［J］. 解剖学报, 2009, 40(1):108-112.
［5］Han J, Liang WM, Hong Y, et al. Heroin withdrawal, detoxication and relapse influence on islet B cell and blood sugar concentration of rat［J］. Chinese Journal of Anatomy, 2013, 36(1): 21-24. (in Chinese)
韩晶, 梁文妹, 洪艳, 等. 大鼠海洛因戒断、脱毒和复吸对胰岛B细胞及血糖浓度的影响［J］.解剖学杂志, 2013, 36(1):21-24.
［6］Pan GSh, Xu GQ, Li ShF. The study of the model of heroin-dependent rat［J］. Journal of Gui Yang Medical College, 1997, 12(Suppl):46. (in Chinese)
潘贵书, 徐国强, 李淑芳. 海洛因成瘾大鼠模型的建立［J］.贵阳医学院学报,1997,12(增刊): 46.
［7］Koob GF, Maldonado R, Stinus L. Neural substrates of opiate withdrawal［J］. Trends Neurosci, 1992, 15(5):186-191.
［8］Ye J,Meng ZQ,Wei ShX, et al. A study on establishm nt of animal models of heroin dependence relapse［J］. Journal of Guang Xi Medical University, 2003, 20(6): 825-832. (in Chinese)
叶峻, 蒙子卿, 韦世秀, 等. 海洛因成瘾复吸动物模型的建立［J］. 广西医科大学学报, 2003, 20: 825-832.
［9］Wookey PJ, Xuereb L, Tikellis C, et al. Amylin in the periphery［J］. Scientific World J, 2003, 24(3):163-175.
［10］Per Westermark. Amyloid in the islets of Langerhans: Thoughts and some historical aspects［J］. Upsala Journal of Medical Sciences, 2011, 116(2): 81-89.
［11］Lutz TA.Control of energy homeostasis by amylin［J］. Cell Mol Life Sci, 2012, 69(12):1947-1965.
［12］Smeltzer M, Scott K, Melhorn S,et al. Amylin blunts hyperphagia and reduces weight and fat gain during recovery in socially stressed rats［J］. Am J Physiol Regul Integr Comp Physiol, 2012, 303(6):R676-682.
［13］Leturque A, BrotLaroche E, Le GallAm M. GLUT2 mutations, translocation, and receptor function in diet sugar managing［J］. J Physiol Endocrinol Metab, 2009, 296(5):985-992.
［14］Nestler EJ, Aghajanian GK. Molecular and cellular basis of addiction［J］. Science, 1997, 278(3):58-63.
［15］Sporer KA. Strategies for preventing heroin overdose［J］. BMJ, 2003, 326(22):442-444.
［16］Appleyard SM, Hayward M, Young JI,et al. A role for the endogenous opioid beta-endorphin in energy homeostasis［J］. Endocrinology, 2003, 144(5):1753-1760.
［17］Kosten TR, George TP. The neurobiology of opioid dependence: implications for treatment［J］. Sci Pract Perspect, 2002, 1(1):13-20.
［18］Okruhlica L, Slezáková S. Weight changes of patients in methadone maintenance treatment during four years period［J］. Cas Lek Cesk, 2012,151(8):389-391.
［19］Smeltzer M, Scott K, Melhorn S, et al. Amylin blunts hyperphagia and reduces weight and fat gain during recovery in socially Stressed rats［J］. Am J Physiol Regul Integr Comp Physiol, 2012, 303(6):R676-682.
［20］Liang WM, Han J, Hu Y,et al. The studies of morphology and functional changes of islet B cells in heroin dependent rats［J］. Acta Anatomica Sinica, 2006, 37(2):215-218. (in Chinese)
梁文妹, 韩晶, 胡赟, 等. 大鼠海洛因依赖期间胰岛B细胞形态学和功能变化的研究［J］.解剖学报, 2006, 37(2):215-218.
［21］Xu J, Zhang L, Chou A, et al. KATP channel-deficient pancreatic β-cells are streptozotocin resistant because of lower GLUT2 activity［J］. Am J Physiol Endocrinol Metab, 2008,294(2): E326-E335.
［22］Stewart J. Stress and relapse to drug seeking: studies in laboratory animals shed light on mechanisms and sources of long-term vulnerability［J］. Am J Addict, 2003, 12(1):1-17.
［23］Pu L, Bao GB, Xu NJ, et al. Hippocampal long-term potentiation is reduced by chronic opiate treatment and can be restored by re-exposure to opiates［J］. J Neurosci, 2002, 22(5):1914-1921.
贵州省社会发展科技攻关计划基金资助;其他(不属于以上基金类别的请自行输入下框)
/
| 〈 |
|
〉 |
