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醒脑静合生脉注射液对大鼠脑出血后凝血酶受体-1表达的影响
张青 蒋艳霞 李国良 王其新 马承泰* 王守彪 姚如永
解剖学报 ›› 2013, Vol. 44 ›› Issue (5) : 630-634.
醒脑静合生脉注射液对大鼠脑出血后凝血酶受体-1表达的影响
Effect of Xing-naojing and Shengmai injection on protease activated receptor-1 expression in rats after intracerebral hemorrhage
目的 探讨醒脑静合生脉注射液对大鼠脑出血后脑组织内凝血酶受体-1（PAR1）表达的影响。方法 将40只雄性SD大鼠随机分为脑出血组（ICH）、生理盐水组（NS）、醒脑静合生脉注射液治疗组（XNJSM）、水蛭素治疗组（HIR），每组10只大鼠。采用自体不凝血注入法建立脑出血模型。术后72h取脑组织，HE染色观察各组血肿周围神经细胞形态学改变，免疫组织化学方法及Western blotting法检测各组血肿周围脑组织PAR1的表达。结果 脑出血后血肿周围脑组织PAR1表达增强，XNJSM组、HIR组脑组织病理形态明显改善，脑组织PAR1表达减少，与ICH组比较差异有显著性（P<0.05）。 结论 醒脑静合生脉注射液可能通过凝血酶受体-1途径有效抑制大鼠脑出血后PAR1蛋白表达，对脑出血后脑组织发挥保护作用。
Objective To investigate the effect of Xing-naojing and Shengmai injection on protease activated receptor-1(PAR1) expression in rat brain tissue after intracerebral hemorrhage (ICH).Methods The ICH model was induced by injecting blood of its own. Forty male SD rats were randomly divided into ICH group (n=10), saline group (NS, n=10), Xing-naojing and Shengmai injection group (XNJSM, n=10), hirudin group (HIR, n=10). Hematoxylin and eosin staining was performed to examine perihematomal neurocyte morphological changes. The expression of PAR1 in perihematomal tissue was examined by immunohistochemistry and Western blotting techniques. Results The expression of PAR1 in perihematomal tissue was increased after ICH. However,compared with ICH group, perihematomal tissue morphological changes were improved obviously. The expression of PAR1 was decreased significantly between XNJSM group and HIR group(P<0.05). Conclusion XNJSM injection may play a neuroprotective effect by inhibiting the expression of PAR1 protein after ICH.
脑出血 / 醒脑静合生脉注射液 / 凝血酶受体-1 / 免疫组织化学 / 免疫印迹法 / 大鼠
Intracerebral hemorrhage / Xing-naojing and Shengmai injection / Protease-activated receptor-1 / Immunohistochemistry / Western blotting / Rat
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山东省中医药管理局基金项目（2005-020）
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