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成年大鼠局灶性脑缺血对SGZ区神经元前体细胞增殖的影响
Effects of transient focal cerebral ischemia on proliferation of neuronal precursors in adult rats subgranular zone
目的 探讨成年大鼠局灶性脑缺血后齿状回颗粒下层（SGZ）神经元前体细胞的增殖改变。方法 大脑中动脉栓塞再灌注（MCAO/R），术后第1天、第2天腹腔注射Brdu，第3天、7天、14天和28天取脑，每个时间组6只SD大鼠，冷冻切片，免疫荧光三标检测SGZ区5-溴脱氧尿嘧啶核苷（Brdu)、DCX、ki67、胶质纤维酸性蛋白（GFAP）及CNP的表达。 结果 （1）MCAO/R后，SGZ区增殖细胞聚集成簇，细胞簇计数从3d开始增加，7d时最多，随后下降；（2）细胞簇内细胞计数从3d开始增加，7d时最多，随后下降；（3）细胞簇中ki67(+)细胞比例从3d开始到28d逐渐下降。结论 MCAO/R引发SGZ区神经元前体细胞经多次分裂以成簇方式增殖，部分子代细胞向神经元分化，部分子代细胞仍保持增殖能力。
Objective To investigate proliferation changes of neuronal precursors in subgranular zone (SGZ) of adult rats after transient focal cerebral ischemia.
Methods Ischemia was induced by occlusion of the middle cerebral artery in rats for 90min. The rats were given intraperitoneal injection of BrdU on the 1st and 2nd day after injury, and then were sacrificed on the 3rd, 7th, 14th and 28th day respectively, 6 rats in time point. The brains were removed and frozen sections were cut. Immunofluorescent staining was applied to observe the expression of Brdu, DCX, ki67, GFAP and CNP in SGZ. Results （1）The Brdu(+) cells assembled into clusters. The number of clusters increased at 3d and reached to peak at 7d，and then decreased gradually.（2）The cells in clusters increased at 3d and reached to peak at 7d, and then decreased gradually.（3）The rate of ki67(+) cells decreased from 3d to 28d. Conclusion MCAO/R induces proliferation of neuronal precursors in SGZ. Some of the progeny cells retain their ability to proliferate, and the others differentiate into neurons.
脑缺血 / 神经元前体细胞 / 颗粒下层 / 免疫组织化学 / 大鼠
Cerebral ischemia / Neuronal precursor / Subgranular zone / Immunohistochemistry / Rat
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