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色钉菇乙酸乙酯提取物对脂多糖损伤的多巴胺能神经元的保护作用
Protective effects of ethyl acetate extract from Chroogomphus rutilus on the degeneration and death of dopaminergic neurons induced by lipopolysaccharides
目的 探讨不同剂量的色钉菇乙酸乙酯提取物对帕金森病（PD）模型小鼠多巴胺能神经元的保护作用。 方法 以昆明小鼠为实验材料，将脂多糖（LPS）定位注射至黑质内复制PD模型。30只小鼠随机分为假手术对照组、LPS 实验对照组、低剂量实验组（LPS+ 100mg/kg）、中剂量实验组（LPS+ 200mg/kg）和高剂量实验组（LPS+ 400mg/kg），每组6只。用免疫荧光方法显示多巴胺能神经元和星形胶质细胞，荧光显微镜下观察、计数黑质内的阳性细胞数。结果 LPS实验对照组小鼠的黑质内，多巴胺能神经元数量少于假手术组（P<0.05），且细胞胞体变圆，突起变短；星形胶质细胞胞体增大, 多呈激活形态。低剂量实验组与中剂量实验组小鼠黑质内的多巴胺能神经元数量多于LPS实验对照组（P<0.05），星形胶质细胞密度降低；高剂量实验组小鼠黑质内的多巴胺能神经元数量与实验组差异无统计学意义（P>0.05）。 结论 低剂量与中剂量的色钉菇乙酸乙酯提取物能够抑制星形胶质细胞的激活，对LPS导致的多巴胺能神经元损伤有显著保护作用。
Objective To investigate the protective effects of different doses of Ethyl Acetate (EA) Extract from Chroogomphus rutilus on the degeneration and death of dopaminergic neurons of the PD mouse which was induced by lipopolysaccharides (LPS). Methods Adult male Kunming(KM) mice were used in the present study, LPS was injected into the substantia nigra compacta(SNc) to duplicate mouse model of PD. A tatal of 30 KM mice were randomly divided into five groups: the sham group, the LPS control group, low dose experimental group(LPS+100mg/kg), medial dose experimental group (LPS+200mg/kg) and high dose experimental group(LPS+400mg/kg), six mice per group. The immunofluorescence methods were used to label the dopaminergic neurons and astrocytes in SN. The dopaminergic positive neurons were counted and analyzed with statistics. Results The dopaminergic neuron numbers in LPS control group were markedly decreased in comparison with sham group (P<0.05), and the dopaminergic neurons appeared ovale, and the neurite was shorter than the sham group. Astrocytes displayed the activated state with larger bodies, thickened and shortened enation in the SN of LPS control group compared with the sham group. The dopaminergic neurons were increased significantly in the low and median dose groups compared with LPS control group(P<0.05), the density of astrocytes was lower. There was no significant difference of dopaminergic neurons between high dose group and LPS control group(P>0.05). Conclusion The low and median groups may protect the degeneration of dopaminergic neurons in SNc by depressing the activity of astrocytes in SNc.
帕金森病 / 脂多糖 / 多巴胺能神经元 / 色钉菇乙酸乙酯提取物 / 立体定位注射 / 小鼠
Parkinson’s disease / Lipopolysaccharides / Dopaminergic neuron / Ethyl acetate extract fromChroogomphus rutilus\ Stereotaxic injection / Mice
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河北省科学技术研究和发展项目No. 11230908D;其他(不属于以上基金类别的请自行输入下框)
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