腹腔注射2-脱氧-D-葡萄糖可诱发大鼠视上核和室旁核神经元表达Fos

赵琳 贾鲲鹏 金晓航 史娟*

doi:10.3969/j.issn.0529-1356.2013.05.003

解剖学报 ›› 2013, Vol. 44 ›› Issue (5) : 602-606. DOI: 10.3969/j.issn.0529-1356.2013.05.003

神经生物学

腹腔注射2-脱氧-D-葡萄糖可诱发大鼠视上核和室旁核神经元表达Fos

赵琳¹贾鲲鹏² 金晓航³ 史娟^3*

作者信息 +

Intraperitoneal injection of 2-DG induces Fos expression in the hypothalamus supraoptic nucleus and paraventricular nucleus of rats

ZHAO Lin¹JIA Kun-peng² JIN Xiao-hang³ SHI Juan ^3*

Author information +

文章历史 +

摘要

目的探讨腹腔注射2-脱氧-D-葡萄糖（2-DG）能否激活大鼠下丘脑视上核（SON）和室旁核（PVN）神经元而表达Fos。方法健康雄性SD大鼠12只，随机分为腹腔注射2-DG组（6只）、生理盐水对照组（3只）及正常对照组（3只）。各自处理后，应用免疫组织化学方法，观察各组下丘脑SON和PVN内Fos表达及其与催产素（OT）和加压素（VP）的双标情况，同时采用ELISA方法对血清中OT和VP的含量进行检测。结果与生理盐水对照组和正常对照组相比，2-DG引发的特异性Fos免疫阳性产物主要集中分布于下丘脑外侧区和穹隆周区，在SON、PVN也有密集表达。SON和PVN内的Fos表达与该区的特异性神经活性物质OT和VP有共存。OT/Fos双标细胞率（双标细胞占OT阳性细胞的百分率）在SON和PVN分别为87.10%、90.57%，明显高于VP/Fos在这两个核团的双标率（双标细胞占VP阳性细胞的百分率，68.42%、76.92%），两者比较差异有统计学意义（P<0.05）。ELISA检测结果显示，2-DG组动物血清中OT和VP水平与对照组相比无明显变化。结论腹腔注射2-DG可激活大鼠下丘脑SON和PVN内OT和VP神经元表达Fos，SON和PVN可能参与2-DG诱导的急性应激反应。

Abstract

Objective To study if intraperitoneal injection of 2-deoxy-D-glucose (2-DG) can functionally activate neurons in the hypothalamus supraoptic nucleus (SON) and paraventricular nucleus (PVN) by induction of Fos expressing.
Methods Twelve healthy male Sprague-Dawley rats were randomly divided into intraperitoneal injection of 2-DG (6 rats), saline control (3 rats) and normal control (3 rats) groups. After the treatment, immunohistochemical staining was employed to observe the expressions of Fos and double labelling of oxytocin (OT) or vasopressin (VP) with Fos in SON and PVN, respectively. The plasmal level of OT and VP was examined by ELISA assay. Results Compared with saline and normal control groups, 2-DG induced specific Fos expression was mainly located in lateral and perifornical areas of hypothalamus. High levels of Fos immunoreactivity were observed in SON and PVN where they double labeled with the specific neuroactive substances OT and VP. The double labeling rates for OT and Fos (the percentage between the number of OT/Fos doublelabeled neurons to that of OT positive neurons) were 87.10% in SON and 90.57% in PVN, significantly higher than that of VP/Fos double labeling rate (68.42% in SON, 76.92% in PVN, P<0.05). In contrast, the plasma level of OT and VP after injection of 2-DG may not show discernible changes to that of control rat. Conclusion Intraperitoneal injection of 2-DG can induce Fos expression in OT and VP neurons in hypothalamic SON and PVN which may be involved in triggering acute stress response induced by 2-DG.

导出引用

赵琳贾鲲鹏金晓航史娟*. 腹腔注射2-脱氧-D-葡萄糖可诱发大鼠视上核和室旁核神经元表达Fos[J]. 解剖学报. 2013, 44(5): 602-606 https://doi.org/10.3969/j.issn.0529-1356.2013.05.003

ZHAO Lin JIA Kun-peng JIN Xiao-hang SHI Juan*. Intraperitoneal injection of 2-DG induces Fos expression in the hypothalamus supraoptic nucleus and paraventricular nucleus of rats[J]. Acta Anatomica Sinica. 2013, 44(5): 602-606 https://doi.org/10.3969/j.issn.0529-1356.2013.05.003

中图分类号： R 322.81

参考文献

［1］Goodson JL,Thompson RR.Nonapeptide mechanisms of social cognition, behavior and species-specific social systems［J］.Curr Opin Neurobiol, 2010,20(6):784-794.
［2］Caldwell HK,Lee HJ,Macbeth AH,et al.Vasopressin: behavioral roles of an “original” neuropeptide［J］.Prog Neurobiol, 2008, 84(1):1-24.
［3］Zenz R,Eferl R,Scheinecker C,et al.Activator protein 1 (Fos/Jun) functions in inflammatory bone and skin disease［J］.Arthritis Res Ther,2008,10 (1):201-210.
［4］Briski KP,Sylvester PW.Hypothalamic orexin-A-immunopositive neurons express Fos in response to central glucopenia ［J］. Neuroreport,2001,12(3):531-534.
［5］Zhao L,Jia KP,Miao NZh,et al.The effect of differential hunger stimuli on orexin-A containing neurons in the perifornical area of hypothalamus［J］.Chinese Journal of Neuroanatomy,2011,27(4):371-376. (in Chinese)
赵琳,贾鲲鹏,苗乃周,等.不同饥饿刺激对下丘脑穹隆周区orexinA 神经元的影响［J］.神经解剖学杂志,2011,27(4):371-376. 
［6］Zhu LL,Onaka T,Zhu ShG. The relation of between oxytocin and vasopressin and stress［J］.Progress in Physiological Sciences, 2002,33 (4):332-335.(in Chinese) 
朱玲玲,尾仲达史,祝世功.催产素和加压素与应激的关系［J］.生理科学进展,2002,33(4):332-335.
［7］Duan XQ, Ju G, Rao ZhR.Induction of Fos oncoprotein expression oxytocin or vasopressin containing neurons in the hypothalamus by substance p in the rat［J］.Acta Anatomica Sinica,1998,29(3):232-236.(in Chinese)
段晓勤,鞠躬,饶志仁.P物质对大鼠下丘脑催产素及加压素免疫反应性神经元Fos癌蛋白的诱导作用［J］.解剖学报,1998,29(3):232-236. 
［8］Lolait SJ,Stewart LQ,Jessop DS.The hypothalamic-pituitary-adrenal axis response to stress in mice lacking unctional vasopressin 1b receptors［J］. Endocrinology, 2007,148(2):9-856.
［9］Liu RR,Cui LB, Lin BW, et al.The effect of differential hypertonic stimuli on the functional status of supraoptic nucleus and paraventricular nucleus in hypothalamus［J］. Chinese Journal of Neuroanatomy,2008,24(5):458-462.(in Chinese)
刘蓉蓉,崔龙彪,李博为,等.不同高渗刺激对下丘脑视上核和室旁核神经元功能状态的影响［J］.神经解剖学杂志,2008,24(5):458-462. 
［10］Sewards TV, Sewards MA. Fear and power-dominance motivation: proposed contributions of peptide hormones present in cerebrospinal fluid and plasma［J］.Neurosci Biobehav Rev,2003,27(3):247-267.
［11］Viero C, Shibuya I, Kitamura N,et al.Oxytocin: crossing the bridge between basic science and pharmacotherapy［J］.CNS Neurosci Ther,2010,16(5):e138-156.
［12］Nakashima T,Noguchi T,Furukawa T,et al.Brain oxytocin augments stress-induced long-lasting plasma adrenocorticotropic hormone elevation in rats［J］.Neurosci Lett, 2002,321(3):161-164.
［13］Mantella R,Vollmer R,Rinaman L,et al.Enhanced corticosterone concentrations and attenuated Fos expression in the medial amygdala of female oxytocin knockout mice exposed to psychogenic stress［J］.Am J Physiol Regul Integr Comp Physiol,2004,287(6):R1494-1504.