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海马微环境对细胞分化和极性化的影响
Effect of hippocampal microenvironment on cells’ differentiation and polarity
目的 观察海马脑片微环境对不同分化程度细胞的极性和分化的影响。方法 将小鼠的海马脑片与5种不同分化程度的细胞（肝癌细胞、正常肝脏细胞、PC12细胞、正常神经细胞和骨髓间充质干细胞）进行共同培养，观察细胞的分化和极性产生情况。结果 与对照组相比，与海马脑片共培养的细胞数量明显减少。共培养的细胞在海马脑片上的分布有一定的规律，主要集中锥体细胞层和颗粒细胞层，其他地方分布均匀，类似于脑片片层化的构筑。海马微环境下低分化的细胞受海马脑片微环境的影响可以产生极性并分化，高分化的细胞不能产生极性。 结论 海马微环境对细胞的极性和分化有促进作用，对生长和分裂有抑制作用，细胞的极性变化与自身和所处的微环境有关，特别对低分化的细胞极性和分化的影响较大。
Objective Our study aimed to investigate the influence of the hippocampal microenvironment on cell’sdifferentiation and polarization. Methods The hippocampal slices were co-cultured with five types of cells (liver cancer cells, normal liver cells, PC12 cells, normal nerve cells and bone marrow mesenchymal stem cells) with various differentiations. The cell differentiation and polarization were observed. Results Compared with the control, the cells distributed with laminar pattern, mainly concentrated in pyramidal cell layer and granule cell layer, with double ‘C’ shape. However, the cells distributed evenly elsewhere. Under the inducement of hippocampal microenvironment, low-differentiated cells underwent polarization and differentiation, but the well-differentiated cells did not. Conclusion The hippocampal microenvironment may promote the cell polarization and differentiation and inhibit the cells’ proliferation. The production of cell polarization and differentiation depends on the differentiated status and the microenvironment.
海马微环境 / 细胞分化 / 细胞极性 / 脑片细胞共培养技术 / 小鼠
Hippocampal microenvironment / Cell differentiation / Cell polarity / Brain slices-cell co-culture techniques / Mouse
［1］Nassif M, Hoppe J, Santin K, et al. β-Amyloid peptide toxicity in organotypic hippocampal slice culture involves Akt/PKB GSK-3βand PTEN ［J］. Neurochem Int, 2007, 50(1)：229-235.
［2］Deng JB, Zhao ShT, Wang GM, et al. Neuropathulogical study on epilepsy in organotypic slice model ［J］. Chinese Journal of Neurology, 2008,41（8）:547-551. (in Chinese)邓锦波,赵善廷,王桂敏,等. 癫痫脑片模型的建立及其病理学研究 ［J］. 中华神经科杂志, 2008（8）,41:547-551.
［3］David LS, Robert DG, Leslie A. Cells A Laboratory Manual ［M］. Beijing:Science Press, 1st edition, 2001:1-7. (in Chinese)
DL斯佩克特, RD戈德曼, LA莱因万德，著, 黄培堂主译. 细胞实验指南 ［M］.北京：科学出版社, 2001:1-7.
［4］Liu YP, Ding HR, He T, et al. A convenient effective and economic method for the mass culture of hepatocytes ［J］.Bulletin of Biology, 2005,1(1):47-48. (in Chinese)
刘友平,丁慧荣,何涛,等. 一种简单、经济、高效的大量肝细胞培养方法［J］. 生物学通报, 2005,1(1):47-48.
［5］Du YF, Zhang Ch. An improvement for culture of neurons of newborn rat hippocampal tissue ［J］. Actac Academiae Medical Qingdao Universitatis, 2002, 38(1):55-56. (in Chinese)
杜怡峰,张晨. 新生大鼠海马神经细胞原代培养方法的改良［J］. 青岛大学医学院学报, 2002, 38(1):55-56.
［6］Li XD, Chen YB, Song XF, et al. Repair of tissue injury in transplantation region of model rats with xenogenic liver transplant by murine bone marrow-derived mesenchymal stem cells ［J］. Chinese Journal of Biologicals, 2008, 21(3): 221-223. (in Chinese)
李秀东,陈玉丙,宋祥福,等. 小鼠骨髓间充质干细胞对异基因肝移植模型大鼠移植区域组织损伤的修复作用 ［J］. 中国生物制品学杂志, 2008, 21（3）: 221-223.
［7］Joan MB, Dorinda R. Ostermann et al. Comparison of two methods to identify live benthic foraminifera: a test between rose bengal and cell tracker green with implications for stable isotope paleoreconstructions ［J］. Paleoceanography, 2006,(21):1029-1037.
［8］Deng JB, ZhShT, Fu X. Hippocampus occurs and the formation of cells and fibers to construct［J］. Chinese Journal of Anatomy, 2012,35(1):119-122. (in Chinese)
邓锦波,赵善廷,符星. 海马发生以及细胞与纤维构筑的形成［J］. 解剖学杂志, 2012,35(1):119-122.
［9］Tostevin F, Howard M. Modeling the establishment of PAR protein polarity in the one-cell C. elegans embryo ［J］. Biophys J, 2008,95(10):4512-4522.
［10］Chen YM, Wang QJ, Hu HS et al. Microtubule affinity-regulating kinase 2 functions downstream of the PAR-3/PAR-6/atypical PKC complex in regulating hippocampal neuronal polarity ［J］. Proc Natl Acad Sci USA, 2006,103(22):8534-8539.
［11］Schwamborn JC, Khazaei MR, Puschel AW. The interaction of mPar3 with the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity ［J］. J Biol Chem, 2007,282(48):35259-35268.
［12］Sottocornola R, Royer C, Vives V et al. ASPP2 binds Par-3 and controls the polarity and proliferation of neural progenitors during CNS development ［J］. Dev Cell, 2010,19(1):126-137.
［13］Zhao J, Qu Y, Mu DZh. Proteins in central nervous system development ［J］. Journal of International Pathology and Clinical Medicine, 2011,31(4):296-300. (in Chinese)
赵婧, 屈艺, 母得志. 极性蛋白与中枢神经系统发育［J］. 国际病理科学与临床杂志, 2011,31(4):296-300.
［14] Fu ZhY, Wang YP. Mechanisms of asymmetric cell divisions during nervous system development［J］. Chinese Journal of Neuroscience, 2004, 20(1):82-88. (in Chinese)
符兆英，王亚萍. 神经系统发育期细胞不对称分裂机制［J］.中国神经科学杂志, 2004, 20(1):82-88.
［15］Rice DS， Sheldon M, D’Areangelo G， et al. Disabled-1 acts downstream of reelin in a signaling pathway that controls laminar organization in the mammalian brain ［J］. Development, 1998, 125(18):3719-3729.
［16］Tissir F, Lambert De Rouvroit C, Sire JY, et al. Reelin expression during embryonic brain development in Crocodylus niloticus［J］. J Comp Neurol, 2003,457(3):250-262.
［17］Hu YH, Xu YH. The establishment of cell polarity ［J］. Chinese Journal of Cell Biology, 2004, 26(4):372-376. (in Chinese)
胡轶红,徐永华. 细胞极性的形成 ［J］. 细胞生物学杂志, 2004, 26(4):372-376.
［18］Takahashi K, Tanabe K, Ohnuki M, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors［J］. Cell, 2007, 131(5):861-872.
［19］Reya T, Morrison SJ, Clarke MF, et al. Stem cells, cancer, and cancer stem cells ［J］. Nature, 2001, 414(6859):105-111.
30670688；30771140；31070952U1204311;国家自然科学基金资助项目;自然科学基金资助项目
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