小鼠肾脏纤维化Masson染色方法的改进

魏胜男 曾翔俊 李汇华*

doi:10.3969/j.issn.0529-1356.2013.04.025

解剖学报 ›› 2013, Vol. 44 ›› Issue (4) : 576-579. DOI: 10.3969/j.issn.0529-1356.2013.04.025

技术方法

小鼠肾脏纤维化Masson染色方法的改进

魏胜男曾翔俊李汇华*

作者信息 +

Improvement of Masson staining in renal fibrosis of mice

WEI Sheng-nan ZENG Xiang-jun LI Hui-hua*

Author information +

文章历史 +

摘要

目的改进Masson三色染色法，以获得较好显示肾脏纤维化的染色方法。方法用Bouin液补充固定组织切片；用-20℃储存的乙醇∶乙酸（2∶1）溶液处理组织；用亮绿代替苯胺蓝；减少亮绿的染色时间。结果明确区分纤维化肾间质和未纤维化的肾间质，胶原纤维呈绿色（用亮绿复染），
胞质和肌纤维呈红色。结论改进后的Masson方法可以将纤维化和非纤维化的肾脏组织进行明显的区分，显微镜下结构显示清晰，着色均匀，取得了较为满意的显色对比效果。

Abstract

Objective To get an improved Masson 3 staining method for staining renal fibrosis. Methods Bouin‘s fluid was used to re-fix the paraffin fixed sections. Ethanol∶acetic acid（2∶1) which was stored at -20℃ was used to treat the sections;
Brilliant green was used to substitute aniline blue. Duration of brilliant green staining was decreased. Results Renal interstitium of fibrosis and non-fibrosis were distinguished. Collagen fibers appeared green (counter staining by brilliant green) and cytoplasm
and muscle fibers were red. Conclusion In improved Masson 3 staining, tissues of fibrosis and non-fibrosis are distinguished by brilliant green, the staining results are stable and uniform. The method demonstrates a satisfied compared staining result.

导出引用

魏胜男曾翔俊李汇华*. 小鼠肾脏纤维化Masson染色方法的改进[J]. 解剖学报. 2013, 44(4): 576-579 https://doi.org/10.3969/j.issn.0529-1356.2013.04.025

WEI Sheng-nan ZENG Xiang-jun LI Hui-hua*. Improvement of Masson staining in renal fibrosis of mice[J]. Acta Anatomica Sinica. 2013, 44(4): 576-579 https://doi.org/10.3969/j.issn.0529-1356.2013.04.025

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