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广西仫佬族线粒体DNA高变Ⅰ区多态性分析
Genetic polymorphism of mitochondrial DNA hypervariable segment Ⅰof Mulam ethnic group in Guangxi
目的 探讨广西仫佬族人群线粒体DNA高变Ⅰ区的多态性特征，了解仫佬族群体的母系遗传结构。方法 收集91例仫佬族无关男性个体的外周血样本，目标序列用引物L15947和R16488进行PCR扩增，用ABI 3730测序仪正反向测序；计算多态性位点、核苷酸多态性、平均配对差异
数目等多态性指标，以及仫佬族与各民族之间的遗传距离，ME法构建遗传进化树。结果 与修正后的剑桥标准序列（rCRS）比对，91例样本的线粒体高变Ⅰ区序列共界定了74种单倍型，核苷酸多态性为0.0188±0.010，平均核苷酸差异为6.618±3.154；遗传距离显示仫佬族与南方各少数
民族及南方汉族的亲缘关系较近，与北方汉族的亲缘关系较远，进化树中仫佬族与南方少数民族聚为一类。结论 仫佬族在母系遗传上属于典型的南方侗傣族群，可能经历过群体扩张或选择效应；高变Ⅰ区序列具有较高的多态性，可用于法医个体识别、民族起源等方面的研究。
Objective To demonstrate the polymorphism of the mitochondrial DNA (mtDNA) hypervariable segment Ⅰ(HVSⅠ) and to clearly understand the maternal genetic structure of Mulam ethnic group in Guangxi. Methods Venous blood samples from 91
unrelated Mulam individuals were collected. HVS I of mtDNA was amplified using primers L15947 and R16488. PCR amplicons were sequenced in both forward and reverse directions using the ABI 3730. Polymorphisms were scored relative to the revised Cambridge
reference sequence (rCRS). The number of polymorphic sites, number of haplotypes, the mean number of pairwise differences and genetic distances between populations were calculated. Polygenetic tree was drawn based on dA distance. Results MtDNA HVSⅠsequences
showed high levels of diversity. Seventy-four haplotypes were identified among the 91 samples. The nucleotide diversity was 0.0188±0.010 and the mean number of pairwise differences was 6.618±3.154. Genetic distances between populations and the cluster
map show that Mulam falls together with southern groups, but away from northern populations. Among southern populations, Mulam clusters were most closely with the Zhuang and Sui. Conclusion Mulam is a typical southern Kam-Tai population, and may experience
population expansion or selection. MtDNA HVS-Ⅰ reveals highly genetic diversities in Mulam, which may have potential use in population genetics and forensic practice.
广西 / 线粒体DNA / 高变Ⅰ区 / 测序 / 遗传多态性 / 聚合酶链反应 / 仫佬族
Guangxi / mt DNA / HVS I / Sequencing / Genetic polymorphism / PCR / Mulam
［1］Wang J, Zheng GQ. A Sketch of Mulam［M］. Beijing: Ethnic Publishing House, 1980: 1-5. (in Chinese)
王均，郑国乔. 仫佬语简志［M］.北京:民族出版社,1980:1-5.
［2］Zheng LB, Lu ShH, Ding B. A study on the physical characteristics of Mulam nationality［J］. Acta Anthropologica Sinica,2006, 25（3）: 242-250. (in Chinese)
郑连彬，陆舜华，丁博.仫佬族体质特征研究［J］. 人类学学报，2006,25(3): 242-250.
［3］Xu L,Deng QY,Li SF, et al.Genetic analysis of Mulao nationality using 15 short tandem repeats［J］. Chinese Journal of Medical Genetics, 2008,25(1):96-100. (in Chinese)
徐林, 邓琼英，李松峰，等.仫佬族15个短串联重复序列的遗传分析［J］. 中华医学遗传学杂志, 2008,25(1):96-100.
［4］Li H. Preliminary analyses of genetic structure of daic populations［C］. Shanghai:Proceedings of The International Symposium of Anthropological Studies, 2002:89-94. (in Chinese)
李辉. 百越族群遗传结构分析的初步思路［C］. 上海:现代人类学国际研讨会论文集,2002:89-94.
［5］Andrews RM, Kubacka I, Chinnery PF, et al. Reanalysis and revision of the Cambridge reference sequence for human mitochondrial DNA［J］. Nat Genet, 1999, 23(2): 147.
［6］Yao YG, Kong QP, Bandelt HJ, et al. Phylogeographic differentiation of mitochondrial DNA in Han Chinese［J］. Am J Hum Genet, 2002, 70(3):635-651.
［7］Yao YG, Nie L, Harpending H, et al. Genetic relationship of Chinese ethnic populations revealed by mtDNA sequence diversit［J］. Am J Phys Anthropol, 2002,118(1):63-76.
［8］Li H, Cai X, Winograd-Cort ER, et al. Mitochondrial DNA diversity and population differentiation in southern East Asia［J］. Am J Phys Anthropol, 2007, 134(4):481-488.
［9］Wen B, Li H, Gao S, et al. Genetic structure of Hmong-Mien speaking populations in East Asia as revealed by mtDNA lineages［J］. Mol Biol Evol, 2005, 22(3):725-734.
［10］Fan HG. Homologous Nationalities：the Origin and Cultural of Zhuang and Tai［M］. Beijing: Ethnic Publishing House, 2007127-128. (in Chinese)
范宏贵.同根生的民族:壮泰各族渊源与文化［M］. 北京：民族出版社，2007:127-128.
［11］Deng QY, Gong JG, Zhou LN, et al. A study on the developmental tendency of subcutaneous fat of Mulao adults in Guangxi［J］. Anatomical Research, 2011,33(1):49-51. (in Chinese)
邓琼英， 龚建古，周丽宁，等. 广西仫佬族成人皮下脂肪发育趋势的研究［J］. 解剖学研究, 2011,33(1):49-51.
［12］Tajima F. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism［J］. Genetics, 1989, 123(3): 585-595.
广西自然科学基金资助项目;自然科学基金资助项目
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