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母源性甲状腺功能亢进对仔鼠心脏甲状腺激素受体mRNA表达的影响
Expression of thyroid hormone receptors in the heart of neonatus rats with maternal hyperthyroidism
目的 探讨母源性甲状腺功能亢进（简称甲亢）对新生仔鼠心脏中甲状腺激素受体的影响。方法 首先对24只雌性Wistar大鼠建立妊娠合并甲亢模型，取新生5d、10d、15d仔鼠心脏进行解剖检查，取部分心脏组织石蜡切片Masson染色，取部分心脏组织采用实时定量PCR法检测甲
状腺激素受体（TR）α1、TRα2、TRβ1 mRNA水平表达量的变化。结果 在心脏组织中TRα1 mRNA的表达量较TRα2、TRβ1的表达量高，且在出生10d的仔鼠心脏（包括甲亢组和对照组）中TRα1mRNA的表达量呈现峰值；与同期对照仔鼠相比，母源性甲亢仔鼠出生5d 与出生10d心肌中TRα1
mRNA的表达量显著上调，出生15d的表达量与对照组相比表达量下调；TRα2在母源性甲亢和对照中差异无统计学意义；TRβ1在甲亢出生5d仔鼠中表达量显著下调，10d组表达量上调，15d组表达量差异无显著性。结论 母源性甲亢会对仔鼠心肌产生影响，引起甲状腺激素受体的差异表达；
这种变化随出生后时间延长而减弱。甲状腺激素受体中TRα1的变化最显著，可能在甲亢引起的心肌损伤中起重要作用。
Objective To detect the expression of thyroid hormone receptors(TR), including TRα1,TRα2 and TRβ1. Methods The animal model of hyperthyroidism was established (population and quantity:24 female Wistar rats). Took the neonatal rat hearts
of day 5, day 10 and day 15 after birth and stained paraffin sections with Masson staining. Detected expression of thyroid hormone receptors including TRα1,TRα2 and TRβ1 by real-time PCR. Results In the cardiac tissue, the TRα1 expression intensities were
higher than TRa2 and TRβ1. In addition, there was a peak in day 10 of neonatal rats both in the hyperthyroidism and the normal ones. In the hyperthyroidism rat, the TRα1 expression intensities in day 5 and day 10 increased significantly,while it decreased
in day 15 in hyperthyroidism. There was no significant difference of TRa2 expression in day 5, day 10 and day 15 between hyperthyroidism and normal. The TRβ1 expression intensity decreased in day 5 in hyperthyroidsm, however, it increased in day 10. Conclusions
The maternal hyperthyroidism may influence the cardiac muscle of the neonatal rats by the different expression of thyroid hormone receptors. Due to the significant change of TRα1, it may play a very important role in myocardial damage of hyperthyroidism.
母源性甲状腺功能亢进 / 甲状腺激素受体 / 实时定量聚合酶链反应 / 仔鼠
Maternal hyperthyroidism / Thyroid hormone receptor (TR) / Real-time PCR / Neonatal rat
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