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14-3-3zeta与p-Bad在T1期非小细胞肺癌中的表达及意义
Expression of 14-3-3zeta and p-Bad proteins in stage T1 non-small cell lung cancer and the significance
目的 观察14-3-3zeta 和p-Bad在T1 期非小细胞肺癌 (NSCLC) 中的表达，探讨两者在非小细胞肺癌发展中的作用及相互关系。方法 取110例非小细胞肺癌及癌旁正常肺组织存档蜡块和50例新鲜NSCLC及癌旁正常肺组织标本，采用免疫组织化学和免疫印迹法（Western blotting）
检测14-3-3zeta 和p-Bad 蛋白的表达。结果 与正常肺组织相比，14-3-3zeta 在NSCLC 中的表达显著增强，与肺癌的分化程度和淋巴结转移有关，而与患者的年龄、性别及病理学分型无关。p-Bad在正常肺组织中轻微表达，而在NSCLC 中表达显著增强。且其表达与患者的年龄、性别及
病理学分型无关，但与癌组织分化程度、淋巴结转移相关。结论 14-3-3zeta蛋白与p-Bad与T1期NSCLC发展、侵袭和转移密切相关。
Objective To observe the expression of 14-3-3zeta and p-Bad in stage T1 non-small cell lung cancer(NSCLC), and to investigate the relationship between the expression of 14-3-3zeta and p-Bad in NSCLC. Methods One hundred and ten paraffin
blocks and 50 fresh samples from NSCLC patients and normal lung were observed. Expression of 14-3-3zeta and p-Bad were detected by immunohistochemistry and Western blotting. Results Up-regulation of 14-3-3zeta was observed significantly in stage T1 NSCLC
in contrast to that in the normal lung. The increased 14-3-3zeta protein level was correlated with differentiation degree and lymph node metastasis, but it was disassociated with age, gender and histological type. Decreased expression of p-Bad was observed
in NSCLC in contrast to that in the normal lung. Expression of p-Bad was related to differentiation degree and lymph node metastasis, but it was disassociated with age, gender and histological type. Conclusion 14-3-3zeta and p-Bad play an important role in
development and progression of NSCLC.
非小细胞肺癌 / 14-3-3zeta / p-Bad / 免疫印迹法 / 人
Non-small cell lung cancer / 14-3-3zeta / p-Bad / Western blotting / Human
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