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核仁素在甲状腺乳头状癌中表达的临床意义
Clinical significance of nucleolin expression in thyroid papillary carcinoma
目的 探讨核仁素（NCL）在甲状腺乳头状癌中的表达及临床意义。方法 采用免疫组织化学SP染色法检测60例正常甲状腺组织与甲状腺乳头状癌中NCL、趋化因子受体-7（CCR7）与基质金属蛋白酶-9（MMP-9）的表达，分析NCL、CCR7与MMP-9的表达与性别、年龄、肿瘤大小与
有无转移的关系以及核仁素与CCR7、MMP-9表达的相关性。结果 NCL在正常甲状腺及甲状腺乳头状癌中的总阳性率分别为0、 100%。NCL在伴随转移组癌组织中表达于细胞核、细胞质与细胞膜；NCL在无转移组癌组织中仅表达于细胞核，差异有统计学意义（P<0.05）。不同年龄、性别、
肿瘤大小的NCL表达率间的差异无统计学意义(P>0.05)。核仁素细胞核外阳性表达与CCR7与MMP-9的阳性表达呈正相关（P<0.01）。结论 NCL在甲状腺乳头状癌中表达，表达部位在转移与非转移组明显不同，且与CCR7、MMP-9表达有密切关系。核仁素可作为区别癌转移与非转移的标志。
Objective To explore the expression of nucleolin in thyroid papillary carcinoma, and discuss its clinical significance.Methods Nucleolin, chemokine receptor 7 (CCR7), matrix metalloproteinase 9 (MMP-9) were detected by immunohistochemical technique
(SP) in 60 cases of normal thyroid tissues and 60 cases of thyroid papillary carcinoma tissues. The relationship among the expressions of nucleolin, CCR7, and MMP-9 and gender, age, or tumor size was analyzed. The correlation between nucleolin and
CCR7/MMP-9 was analyzed. Results The expression rate of nucleolin in normal thyroid and thyroid papillary carcinoma tissues was 0, 100% respectively. The expression of nucleolin in thyroid papillary carcinoma with lymph node metastasis was mainly located
in nucleus, cytoplasm and cell membrane while the expression of nucleolin was only located in nucleus in thyroid papillary carcinoma tissues without lymph node metastasis. Differences were statistically significant（P<0.05）. No significant correlation
was found between the expression of nucleolin and gender, age or tumor size (P>0.05). In thyroid papillary carcinoma, the extra nuclear expression of nucleolin was positively correlated with the expression of CCR7 and MMP-9（P<0.01）. Conclusion Our
study suggests that nucleolin can express in thyroid papillary carcinoma and the distribution of nucleolin differs between metastatic carcinoma tissues and nonmetastatic carcinoma. Nucleolin is closely associated with CCR7 and MMP-9. NCL can be
used as an important indicator for cancer metastasis.
核仁素 / 趋化因子受体-7 / 基质金属蛋白酶-9 / 甲状腺乳头状癌 / 免疫组织化学 / 人
Nucleolin / Chemokine receptor 7 / Matrix metalloproteinase 9 / Thyroid papillary carcinoma / Immunohistochemistry / Human
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ZR2009CZ014；ZR2010HM047;省自然科学基金资助项目
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