欢迎访问《解剖学报》官方网站！今天是
English
雌激素对大鼠红核神经元保护作用的体视学定量
Neural protective effect of estrogen on the rat red nucleus by dissector quantitative analysis
目的 观察雌激素（E2）替代治疗对去卵巢SD大鼠脊髓损伤后红核神经元逆行性损伤的影响。方法 成年雌性SD大鼠80只, 随机分为正常组、红核脊髓束(RST)损伤组、E2替代治疗组、雌激素受体拮抗剂（ICI）治疗组和E2+ICI联合治疗组。SD大鼠去势后1周采用选择性切断脊髓C3～C4左侧背外侧索制作单侧红核脊髓束（RST）横断损伤模型，给予不同条件治疗后1周、2周和4周对各组大鼠采用前肢支撑探测实验进行行为学评价，用红色荧光金（FR）逆行荧光示踪及体视学定量分析法，观察红核神经元的形态及数目变化。结果 前肢支撑探测实验结果显示，各时间点E2替代治疗组的左前肢使用率均高于除正常组外其他各组，但无统计学意义（P >0.05）；形态学检测结果可见各组大鼠中脑右侧FR阳性红核神经元数目除正常组外均有不同程度减少，尤以4周时最为明显。E2替代治疗组右侧中脑FR阳性红核神经元与RST损伤组、ICI治疗组和E2+ICI 联合治疗组相比胞体饱满、轮廓清晰、突起长，体视框计数结果显示，E2替代治疗组红核FR阳性神经元数目明显多于其余各组（P<0.05），但RST损伤组、ICI治疗组和E2+ICI联合治疗组之间右侧中脑FR阳性红核神经元数目未见明显差异（P>0.05）。结论 大鼠脊髓横断损伤后中脑红核神经元发生逆行性损伤，E2替代治疗可减轻脊髓损伤引发的继发性红核神经元损伤。
Objective To investigate the protective effect of estrogen(E2) on red nucleus after spinal cord injury (SCI) inthe ovarectomized rat. Methods Eighty adult female rats were selected in this experiment and randomly divided into the normal group, rubrospinal tract injury group, estrogen replacement group, inhibitor of estrogen receptor (ICI) treatment group and E2+ICI cooperative treatment group. Sole rubrospinal tract injury model was established by cutting off the left dorsolateral funiculus at C3-C4 segment of rat spinal cord on one week after ovariectomy operation. The animal behavior was evaluated with forelimb probing experiment, and FR positive neurons were counted and analyzed on 1 week, 2 weeks and 4 weeks by using disecter after different treatments on animals. Results The forelimb probing test showed a higher use rate of left forelimb in the E2 replacement treatment group than that of other groups, but with no statistical significance (P>0.05). Except the normal group, the number of neurons in right red nucleus of rat midbrain decreased, especially on 4 weeks. The FR positive neurons of E2 replacement group had a more remarkable cell body, clearer outline and longer processes compared to the neurons in the RST injury group, ICI treatment group and E2+ICI cooperative treatment group. The stereological frame illustrated that more FR positive neurons survived in the E2 replacement group (P<0.05), but with no significant changes (P>0.05) among the RST injury group, ICI treatment group and E2+ICI cooperative treatment group. Conclusion The E2 replacement treatment may release red nucleus neuron retrograde injury and promote the neuron survival after rat SCI.
Estrogen / Red nucleus / Retrograde tracing / Disector / Rat
［1］Hauben E, Butovsky O, Nevo U, et al. Passive or active immunization with myelin basic protein promotes recovery from spinal cord contusion［J］. Neuroscience, 2000, 20(17): 6421-6430.
［2］Webb AA, Muir GD. Unilateral dorsal column and rubrospinal tract injuries affect overground locomotion in the unrestrained rat［J］. Eur J Neurosci, 2003, 18(2):412-422.
［3］Storer PD, Houle JD. Beta II-tubulin and GAP-43 mRNA expression in chronically injured neurons of the red nucleus after a second spinal cord injury［J］. Exp Neurol, 2003, 183(2): 537-547.
［4］Guo J, Duckles SP, Weiss JH, et al. 17β-Estradiol prevents cell death and mitochondrial dysfunction by an estrogen receptor-dependent mechanism in astrocytes after oxygen-glucose deprivation/reperfusion［J］. Free Radic Biol Med, 2012, 52(11-12):2151-2160.
［5］Napoli I, Noon LA, Ribeiro S, et al. A central role for the ERK-signaling pathway in controlling Schwann cell plasticity and peripheral nerve regeneration in vivo［J］. Neuron, 2012, 73(4):729-742.
［6］Li YF. 3D-reconstruction and stereological analysis of the brain structure of the Gekko japonicus［D］. Jiangsu: Nantong University, 2011:1-60. (in Chinese)
李耀富. 多疣壁虎脑部结构的三维重建及体视学分析［D］. 江苏: 南通大学, 2011:1-60.
［7］Gundersen HJG, Bagger P, Bendtson TF, et al. The new stereological tool: disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis［J］. Acta Pathol Microbiol Immunol Scand, 1988, 96(10): 857-881.
［8］Hicks TP, Onodera S.The mammalian red nucleus and its role in motor systems, including the emergence of bipedalism and language［J］. Prog Neurobiol, 2012, 96(2):165-175.
［9］Lee JH, Tigchelaar S, Liu J, et al. Lack of neuroprotective effects of simvastatin and minocycline in a model of cervical spinal cord injury［J］. Exp Neurol, 2010, 225(1):219-230.［10］Tobias CA, Han SS, Shumsky JS, et al. Alginate encapsulated BDNF-producing fibroblast grafts permit recovery of function after spinal cord injury in the absence of immune suppression［J］. J Neurotrauma, 2005, 22(1):138-156.
［11］Xiao M, Klueber KM, Lu C, et al. Human adult olfactory neural progenitors rescue axotomized rodent rubrospinal neurons
and promote functional recovery［J］. Exp Neurol, 2005,194(1):12-30.
［12］Ma QT, Zeng YSh, Zhang W, et al. Effects of ganoderma spore and l-nna on the neuronal survival and axonal regeneration in clarke,s nucleus and red nucleus after rat spinal cord injury［J］. Acta Anatomica Sinica, 2005, 36(6):597-603. (in Chinese)
马钦桃, 曾园山, 张伟, 等. 灵芝孢子和一氧化氮合酶抑制剂对大鼠脊髓损伤后背核、红核神经元存活及轴突再生的影响［J］. 解剖学报, 2005, 36(6):597-603.
［13］Chen A, Zhang JW, Wu XQ, et al. Protective effects of Buyang Huanwu Decoction on neurons after rubrospinal tract transection［J］. Chinese Journal of Clinical Anatomy, 2007, 25(6): 665-668. (in Chinese)
陈安, 张建伟, 伍校琼, 等. 补阳还五汤对红核脊髓束横断后神经元的保护作用［J］. 中国临床解剖学杂志,2007, 25(6): 665-668.
［14］Zhang GQ, Yan JF, Fan LH. Exprimental study on neuron protection in red nucleus by transplanting BDNF gene modified neuroal stem cells after spinal injury［J］. Shandong Medical Journal, 2006, 46(35): 21-22. (in Chinese)
张国庆, 燕景锋, 樊立华. BDNF基因修饰神经干细胞移植对脊髓损伤后红核神经元作用的实验研究［J］. 山东医药, 2006, 46(35): 21-22.
［15］Yang ZhW. A new tool of biosterology——the optical disector［J］. Chinese Journal of Sterology and Image Analysis, 1998, 3(1): 50-54.(in Chinese)
杨正伟. 生物体视学新工具——光学体视框［J］. 中国体视学与图像分析, 1998, 3(1): 50-54.
［16］Weibel ER, Elias H. Quantitative Methods in Morphology/Quantitative Methoden in der Morphologie［M］. Berlin: Springer Berlin Heidelberg, 1967: 89-98.
［17］Krasnoperov RA. Stereological probing and its use in modern research［J］. Morfologiia, 2007, 131(1):73-78.
［18］Miki T, Satriotomo I, Li HP, et al. Application of the physical disector to the central nervous system: estimation of the total number of neurons in subdivisions of the rat hippocampus［J］. Anat Sci Int, 2005, 80(3):153-162.
市自然科学基金资助项目
/
| 〈 |
|
〉 |
