大鼠2型糖尿病早期肾小管细胞聚核糖性死亡

李淑玲 阎文柱*

doi:10.3969/j.issn.0529-1356.2013.03.023

解剖学报 ›› 2013, Vol. 44 ›› Issue (3) : 409-412. DOI: 10.3969/j.issn.0529-1356.2013.03.023

组织学胚胎学发育生物学

大鼠2型糖尿病早期肾小管细胞聚核糖性死亡

李淑玲¹阎文柱^2*

作者信息 +

Parthanatos involved in tubular damages of the kidney in rats with type 2 diabetics

LI Shu-ling¹ YAN Wen-zhu ^2*

Author information +

文章历史 +

摘要

目的探讨2型糖尿病大鼠肾小管细胞聚核糖性死亡的形态学特点。方法 20只雄性SD大鼠分为正常组和2型糖尿病组，在光学显微镜和
电子显微镜下观察两组大鼠肾小管细胞结构的变化，用免疫组织化学染色、免疫印迹方法检测多聚二磷酸腺苷核糖聚合酶-1（PARP-1）蛋白的
表达。结果 2型糖尿病早期肾小管细胞内有大量糖原聚集，细胞肿胀，部分细胞坏死，主要为空泡样坏死。免疫组织化学染色显示，2型糖尿病
肾小管上皮细胞PARP-1表达阳性。免疫印迹法显示2型糖尿病肾小管细胞中PARP-1蛋白表达增强。结论 2型糖尿病早期肾小管细胞发生了坏死，
聚核糖性死亡（PARP-1介导的细胞死亡）参与了2型糖尿病早期肾小管细胞的损伤。

Abstract

Objective To observe the parthanatos involved in tubular cell damages of kidney in rats with type 2 diabetics.
Methods Animal models of type 2 diabetics were established. Light microscopy, electron microscopy, immunohistochemistry and
Western blotting were used in this study. Results A large number of glycogen accumulation, cell swelling, and partial necrosis,
mainly for the vacuolar necrosis were seen in early type 2 diabetes renal tubular cells. Immunohistochemical staining of PARP-1
showed that the positive cells were found in epithelium of renal tubules of type 2 diabetic kidney. Compared to the control
kidney, PARP-1 expressed much stronger by immunoblot analysis. Conclusion Necrosis and parthanatos are involved in renal tubule
damages induced by type 2 diabetes in rats.

导出引用

李淑玲阎文柱*. 大鼠2型糖尿病早期肾小管细胞聚核糖性死亡[J]. 解剖学报. 2013, 44(3): 409-412 https://doi.org/10.3969/j.issn.0529-1356.2013.03.023

LI Shu-ling YAN Wen-zhu*. Parthanatos involved in tubular damages of the kidney in rats with type 2 diabetics[J]. Acta Anatomica Sinica. 2013, 44(3): 409-412 https://doi.org/10.3969/j.issn.0529-1356.2013.03.023

参考文献

［1］David KK, Andrabi SA, Dawson TM, et al. Parthanatos: a messenger of death［J］. Front Biosci, 2009, Jan 1(14):1116-1128.
［2］Drel VR, Xu W, Zhang J, et al. Poly(adenosine 5 -diphosphate-ribose) polymerase inh ibition counteracts multiple manifestations of experimental type I diabetic nephropathy［J］. Endocrinology,2009,150(12):5273-5283.
［3］Li XM, Guo M. Morphology of principle cells and expression of aquaporin-2,-3 in collecting duct during mouse kidney development［J］. Acta Anatomica Sinica, 2011,42(3):389-393.(in Chinese)
李晓明, 郭敏. 小鼠肾集合管发育中主细胞的形态学变化及水通道蛋白-2和-3的表达［J］.解剖学报, 2011,42(3):389-393. 
［4］Chen G, Lu FE, Xu LJ. Effects of Huang lian Jie du Decoction on glucose transporter 4 intarget tissues of type 2 diabetic rats［J］.Journa1of Chinese Integrative Medicine, 2007,5(4): 412-415. (in Chinese)
陈广，陆付耳，徐丽君.黄连解毒汤对2型糖尿病大鼠靶组织葡萄糖转运子4的影响［J］.中西医结合学报,2007,5（4）:412-415. 
［5］Fu WL, Fu XM, Zhong MR, et al. Effects of sericine on growth hormone/insulin-like growth factor-1 axis of testis in type 2 diabetes mellitus rats［J］. Acta Anatomica Sinica, 2011, 42 (1): 104-109. (in Chinese)
付文亮，付秀美，钟美蓉，等. 丝胶对2型糖尿病大鼠睾丸生长激素/胰岛素样生长因子-1轴的作用［J］.解剖学报, 2011, 42 (1): 104-109. 
［6］Kroemer G, Galluzzi L, Vandenabeele P, et al. Classification of cell death: recommendations of the Nomenclature Committee on Cell Death ［J］. Cell death Differ, 2009, 16(1):3-11.
［7］Thomas MC, Burn WC, Cooper ME. Tubular changes in early diabetic nephropathy［J］. Adv Chronic Kidney Dis, 2005,12(2):177-186.
［8］Magri CJ, Fava S. The role of tubular injury in diabetic nephropathy［J］. Eur J Intern Med, 2009, 20(6):551-555. 
［9］Koh DW, Dawson TM, Dawson VL. Mediation of cell death by poly(ADP-ribose) polymerase-1 ［J］. Pharmacol Res,2005, 52(1):5-14.
［10］Alano CC，Garnier P,Ying W,et al. NAD depletion is necessary and sufficient for PARP-1 mediated neuronal death ［J］. J Neurosci,2010, 30(8):2967-2978.
［11］Luo X, Kraus WL. On PAR with PARP:cellular stress signaling through poly(ADP) and PARP-1［J］. Genes Dev, 2012, 26(1):417-432.
［12］Galluzzi L, Vitale I, Abrams JM, et al. Molecular definitions of cell death subroutines: recommendations of the Nomenclature Committee on Cell Death 2012［J］. Cell Death Differ,2012,19(1):107-120.