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生殖细胞核因子及Oct-4在P 19细胞系诱导分化中的表达
王菊 熊承良 高启云 李红钢 王苗 赵凯 周宗瑶*
解剖学报 ›› 2013, Vol. 44 ›› Issue (3) : 349-352.
生殖细胞核因子及Oct-4在P 19细胞系诱导分化中的表达
Expression of germ cell nuclear factor and Oct-4 before and after in vitro differentiation of mouse P19 Cell line
目的 体外培养未成熟畸胎瘤细胞（P19细胞）并诱导分化，检测生殖细胞核因子（GCNF）及Oct-4在P 19细胞诱导分化前后的表达。
方法 用全反式维甲酸（ATRA）诱导P 19细胞分化，通过间接免疫荧光染色和RT-PCR方法检测GCNF及Oct-4在P 19细胞诱导分化前后的表达。
结果 Oct-4在P 19细胞诱导前呈强阳性表达，诱导2 d后表达下降；而GCNF在P 19细胞诱导前呈阴性表达，诱导2 d后呈阳性表达。
结论 GCNF参与了恶性生殖细胞肿瘤的体外分化。GCNF可能是通过下调Oct-4基因的表达参与了恶性生殖细胞肿瘤的分化。
Objective To detect the expressions of Oct-4 and germ cell nuclear factors(GCNF) before and after inducing P19
cells to differentiation. Methods The P19 cells were induced to differentiation with ATRA treatment.The immunofluorescence
staining and RT-PCR techniques were used to detect the expression of GCNF and Oct-4 before and after differentiation of mouse
P19. Results The intensities for Oct-4 immunofluorescence positive staining were stronger in non-differentiated P19 cells than
that in the differentiated P19 cells. The intensities for GCNF immunofluorescence positive staining were weaker in non-differentiate
P19 cells than that in the differentiated P19 cells. Conclusion GCNF is involved in the in vitro differentiation progress of
the malignant germ cell tumors. GCNF be inhibited through the Oct-4 gene expression in vitro differentiation of P19 cells.
Oct-4 / 生殖细胞核因子 / P19细胞 / 分化 / 间接免疫荧光 / 反转录-聚合酶链反应
Oct-4 / Germ cell nuclear factor / P19 cell / Differentiation / Indirect immunofluorescence / RT-PCR
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