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小鼠晶状体发育过程中细胞增殖及突触素蛋白的表达
Cell proliferation and synaptophysin expression in the developing mouse lens
目的 探讨小鼠晶状体细胞增殖、细胞周期蛋白（cyclin) D1和突触素（SYN）的表达情况。方法 各日龄小鼠共150只，HE染色和
4’6-二脒基-2-苯基吲哚（DAPI）染色，在光镜下观察小鼠晶状体的一般结构；用抗细胞增殖核抗原（PCNA）免疫荧光法以及5′-溴脱氧尿嘧
啶核苷（BrdU）技术标记晶状体增殖细胞；用免疫荧标记标价法检测细胞周期蛋白cyclin D1和SYN在晶状体的表达。结果 孕8d（E8）左右，
晶状体由最初的晶状体板分化而来，是由单层柱状上皮细胞围成的空泡，空泡再进一步分化，被原始晶状体纤维所替代，逐渐形成晶状体；
BrdU和PCNA的阳性细胞主要分布在晶状体上皮细胞的前囊中央区，但出生10d（P10）以后BrdU阳性细胞已不在晶状体上皮细胞分布，而PCNA持
续表达。Cyclin D1阳性细胞在胚胎期主要在晶状体上皮细胞的赤道部表达，出生早期在晶状体上皮细胞的前囊中央区，出生5d（P5）以后在赤
道前区分布，赤道部有少量阳性细胞；在晶状体发育过程中，SYN可以在晶状体基质中和赤道部胞体中发现。结论 细胞增殖对晶状体的发育和
修复起着重要的作用；cyclin D1可能参与调控赤道部细胞的消失，因此保持晶状体的透明度；SYN在晶状体的表达说明了它对晶状体的透明有
着重要的作用，通过调控钙离子内流入胞体和基质中。
Objective To study cell proliferation and the expression of cell cycle protein (cyclin D1) and synaptophysin
(SYN) in mouse lens. Methods A total 150 mice were used in the study. HE staining and DAPI staining were performed out to
observe the general structure of the mouse lens. Proliferating cell nuclar antigen (PCNA) immunofluorescent staining and
5-bromodeoxyuridine (BrdU) assay were used to mark the proliferative cells in lens. The expression of cyclin D1 protein and
synaptophysin in the lens were investigated with cyclin D1 and synaptophysin immunofluorescent staining. Results At about E8,
the lens were evolved from the lens placode with single columnar epithelium. Then, the hollow lens bulb developed further
and was filled with numerous fibroblasts, which gradually formed lens. BrdU and PCNA-positive cells were mainly distributed
in the epithelium of anterior capsule. However, P10 afterward, BrdU positive cells disappeared, while PCNA continued to express
in the anterior epithelial cells. Cyclin D1-positive cells were mainly distributed in the epithelium of anterior capsule and
the equator of the lens in the embryonic days and early postnatal days. After P5, cyclinD1-positive cells were mainly located
in the pre-equatorial area, and only few cyclin D1 positive cells were found in the equator. Synaptophysin protein expressed
in the matrix and some cell bodies of equator during the development.Conclusion The cell proliferation plays an important role
during the lens development and lens repair. Cell cycle proteins, cyclin D1 is probably involved in regulating the cells’disappear
in the equator, therefore the lens’s transparency can be kept. Synaptophysin’s expression in lens has important function in
lens’s transparency, through its regulation to calcium flow in cell bodies and matrix of lens.
晶状体 / 细胞增殖 / 细胞周期蛋白 / 突触素蛋白 / 免疫荧光 / 小鼠
Lens / Cell proliferation / Cell cycle protein / Synaptophysin protein / Immunofluorescence / Mouse
［1］ Shi HH, Guan HJ. The influence of IOL design on posterior capsule opacification［J］.International Journal of Ophthalmology, 2004, 4(5): 882-886. (in Chinese)
石海红，管怀进. 人工晶状体的设计对后囊膜混浊的影响［J］. 国际眼科杂志，2004，4(5)：882-886.
［2］ Jia YQ, Sun HM. Development trend of the intraocular lens［J］. Chinese Journal of Ophthalmology, 1990,26(6): 323-325.
佳袁琴，孙惠敏.人工晶体的发展趋势［J］. 中华眼科杂志，1990，26(6)：323-325.
［3］Znsman I, Reifen R, Livni O. Role of apoptosis, proliferating cell nuclear antigen and p53 protein in chem ically induced colon cancer in rats fed corncob fiber treated with the fungus pleurotuso streatus ［J ］. A nticancer Res, 1997,17 (3c) : 2105-2113.
［4］ Zang JF, Niu YL, Demg JB, et al. Neocortical lamination and cell cycle in the mouse［J］. Acta Anatomica Sinica, 2012, 43(1):19-27. (in Chinese)
臧建峰，牛艳丽，邓锦波，等. 小鼠大脑新皮质片层化形成过程和细胞周期变化［J］. 解剖学报，2012，43(1)：19-27.
［5］ Yang Ch. Some research of synaptophysin［J］. Henan Medical Research, 2004, 13(1): 85-89. (in Chinese)
杨春.突触素的若干研究［J］. 河南医学研究，2004，13(1)：85-89.
［6］ Xia ZhX, Wu Y, Feng ZhZh, et al. Establishment of lens development model in Kunming mouse［J］. Journal of Clinical Ophthalmology, 2011, 19(5): 458-460. (in Chinese)
夏朝霞，吴艺，冯志贞，等. 小鼠晶状体连续发育模型的建立［J］. 临床眼科杂志，2011，19(5)：458-460.
［7］ Cai ZhM, Chen RH, Liang P, et al. Light Microscopic and electron microscopic observation on the developing course of lens capsule and lens epithelium in fetus［J］. Chinese Journal of Anatomy, 1993,16(2):95-98. (in Chinese)
蔡兆明，陈瑞华，梁平，等.胎儿晶状体囊及晶状体上皮发育过程的光、电镜观察［J］. 解剖学杂志，1993，16(2)：95-98.
［8］ Wang XJ, Yuan ShL, Huang RM. An Observation of the effect of tanshinone on cancer cell proliferation by Brdu and PCNA Labeling［J］. Journal of West China University of Medical Sciences, 1996, 27(4): 388-391. (in Chinese)
王修杰，袁淑兰，黄韧敏. BrdU和PCNA标记观察丹参酮对癌细胞增殖的影响［J］. 华西医科大学学报，1996，27(4)：388-391.
［9］ Yang ZhL, Li YG, Liu GL, et al. Expression of cyclin D1, cdk4, p16 and Rb in gallbladder carcinoma and its significance［J］. Chinese Journal of Hepatobiliary Surgery, 2001, 2(7):84-86. (in Chinese)
杨竹林，李永国，刘国利,等. 胆囊组织中cyclin D1，cdk4，p16，Rb 表达及意义［J］. 中华肝胆外科杂志，2001，2(7)：84-86.
［10］Coqueret O.Linking Cyclins to transcriptional control［J］.Gene，2002，299(1-2): 35-55.
［11］ Liu J，Li GY，Xu DCh, et al. Expression and significance of synaptophysin in the oculomotor nucleus in midbrain of fetus［J］. Chinese Journal of Neuroanatomy, 2011, 27(2): 198-202. (in Chinese)
刘靖，李国营，徐达传,等. 突触素在中脑动眼神经核发育中的表达及意义［J］. 神经解剖学杂志，2011，27(2)：198-202.
［12］Bitel CL, Nora I. Perrone-Bizzozero NI, Frederikse PH. HuB/C/D, nPTB, REST4, and miR-124 regulators of neuronal cell identity are also utilized in the lens［J］. Mol Vis,2010,16:2301-2316.
［13］ Yan HL, Yu XM. Ocular Physiology［M］. Beijing:People’s Medical Publishing House, 2001: 69-74. (in Chinese)
阎洪禄，于秀敏.眼生理学［M］. 北京：人民卫生出版社，2001：69-74.
［14］ Li WH, Kang ZF. Modern medicine and traditional medicine research on cataract and thinking［J］. International Journal of Ophthalmology, 2010, 10(2): 288-291. (in Chinese)
李伟华,亢泽峰. 现代医学与传统医学对白内障的研究进展与思考［J］.国际眼科杂志，2010，10(2)：288-291.
30670688；30771140；31070952;国家自然科学基金资助项目;自然科学基金资助项目
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