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扬子鳄大脑胚后发育中脑源性神经营养因子和神经营养因子-3的表达
Expression of brain-derived neurotrophic factor and neurotrophin-3 in post-embryonic development of cerebral cortex of the Chinese alligator
目的 探讨脑源性神经营养因子(BDNF)和神经营养因子3(NT-3)在扬子鳄大脑胚后发育中的时序表达。方法 用免疫组织化学染色和
Western blotting法观察BDNF和NT-3在0岁、1岁和2岁的扬子鳄大脑皮质中的定位和含量变化。结果 免疫组织化学显示，0岁扬子鳄大脑皮质
区内4层均可见BDNF阳性细胞，随着年龄增加，BDNF阳性细胞数量逐渐增多，到2岁时BDNF阳性细胞胞体增大，突起增多，主要集中在细胞层和
外网状层移行区。这与Western blotting检测结果一致。0岁扬子鳄大脑皮质区内可见NT-3阳性细胞，主要集中在细胞层和外网状层。到1岁时，
NT-3阳性细胞逐渐增多，在外网状层可见大量密集的阳性细胞。2岁时，外侧的分子层中NT-3阳性细胞增多，且细胞突起随着年龄增加而出现
增多。这一结果与Western blotting检测一致。结论 BDNF和NT-3参与了扬子鳄胚后2年内大脑皮质区神经细胞的生长发育。
Objective To investigate sequential changes of brain-derived neurotrophic factor(BDNF) and neurotrophin-3(NT-3)
expressed in the post-embryonic development in cerebral cortex of Alligator sinensis. Methods Immunohisto chemistry technique
and Western blotting assay were used to detect the temporal expression concerning its variation at location and content of BDNF
and NT-3 in the cerebral cortex of the Chinese alligator by postembryonic age of 0 year(newly hatched), 1 year and 2 years.
Results Immunohistochemical test showed that BDNF-positive cells were seen at the 4 layers of cerebral cortex from the alligator
aged 0 and increased with ages. By 2 years, the cell body for BDNF-positive cells gradually expanded, with the increased nerve
processes that were mostly observed at the transition zone of the cell layer and the outer plexiform layer, which was consistent
with Western blotting findings. NT3-positive cells were also seen in cerebral cortex of the newly hatched alligator, concentrated
in the cell layer and the outer plexiform layer, and gradually increased by 1 year of age, with more intensive positive cells in
the outer plexiform layer. By 2 years, NT3-positive cells increased in the molecular layer at lateral zone, and similarly, cell
projections tended to increase with ages, which agreed with the results by Western blotting assay. Conclusion Both BDNF and NT-3
are involved in the post-embryonic development in cerebral cortex of Chinese alligator within 2 years of age.
大脑 / 胚后发育 / 脑源性神经营养因子 / 神经营养因子-3 / 免疫组织化学 / 免疫印迹法 / 扬子鳄
Cerebral cortex / Postembryonic development / Brain-derived neurotrophic factor / Neurotrophin-3 / Immunohistochemistry / Western blotting / Chinese alligator
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