欢迎访问《解剖学报》官方网站！今天是
English
不同发育时期鸡胚脊髓内背侧抑制性轴突导向蛋白的表达和神经嵴细胞迁移之间的相关性
苏玉红* 张三兵 崔慧先 康林 王磊 杜鹃 袁世君
解剖学报 ›› 2013, Vol. 44 ›› Issue (3) : 304-307.
不同发育时期鸡胚脊髓内背侧抑制性轴突导向蛋白的表达和神经嵴细胞迁移之间的相关性
Correlation of draxin expression and neural crest migration in chick embryonic spinal cord at different developmental stage
目的 探讨不同发育时期的鸡胚脊髓内背侧抑制性轴突导向蛋白（draxin）表达与神经嵴迁移之间在空间分布上的相关性。方法 选
取HH 15-16、HH19-20阶段鸡胚各10只，应用原位杂交、免疫组织化学等方法，观察各阶段鸡胚脊髓内draxin的表达部位及神经嵴细胞迁移路
径，同时比较两者在空间分布上的相关性；应用活组织切片与碱性磷酸酶标记的draxin融合蛋白（draxin-AP）体外孵育的方法，检测迁移路
径内的神经嵴细胞是否具有与draxin蛋白直接结合的能力。结果 随着发育时间的推移，鸡胚脊髓内draxin的表达和神经嵴迁移都具有明显的
由颅侧向尾侧渐进性分布的特性，且draxin的表达部位位于神经管背侧、顶板和背侧生皮肌节之间，这些部位恰均位于神经嵴迁移路径的周围；
同时迁移路径内的神经嵴细胞具有与draxin蛋白体外直接结合的能力。结论 draxin表达在神经嵴迁移路径周围且部分神经嵴具有与draxin蛋白
体外直接结合的能力，推测draxin可能在鸡胚脊髓神经嵴迁移过程中发挥重要的调节作用。
Objective To examine the correlation of draxin expression and neural crest migration in chick embryonic spinal
cord at different developmental stages.Methods Ten chick embryos at different stages were used. In situ hybridization and
immunohistochemistry were used to observe the localization of draxin expression, the migration pathway of neural crest cell-the
spatial correlation of draxin expression and the neural crest migration. Living tissue incubation with draxin alkaline phosphatase
(draxin-AP) recombinant protein was used to check the binding ability of migrating neural crest with draxin protein. Results With
development, the draxin expression and neural crest migration had a very similar anterior-posterior gradient in chick embryonic
spinal cord. Draxin was expressed in the dorsal neural tube, roof plate and dorsal tip of dermomyotome which surrounded the
migration pathway of neural crest cells. We also found that portion of migrating neural crest cells bound to draxin-AP protein
directly. Conclusion Draxin is expressed in the tissues surrounding the neural crest migration pathway and portion of migrating
neural crest cells binds to draxin protein directly. We conclude therefore that draxin may be involved in the regulation of neural
crest cell migration in chick embryonic spinal cord.
背侧抑制性轴突导向蛋白 / 脊髓 / 神经嵴 / 迁移 / 原位杂交 / 免疫组织化学 / 鸡胚
Draxin / Spinal cord / Neural crest / Migration / In situ hybridization / Immumohistochemistry / Chick embryo
［1］Stemple D L, Anderson D J. Isolation of a stem cell for neurons and glia from the mammalian neuralcrest［J］. Cell, 1992, 71(6):973-985.
［2］Islam SM, Shinmyo Y, Okafuji T, et al. Draxin, a repulsive guidance protein for spinal cord and forebrain commissures［J］. Science, 2009, 323(5912): 388-393.
［3］Hamburger V, Hamilton HL. A series of normal stages in the development of the chick embryo.1951［J］. Dev Dyn, 1992, 195(4):231-272.
［4］Schaeren-Wiemers N, Gerfin-Moser A. A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled Crna probes［J］. Histochemistry, 1993,100(6):431-440.
［5］Okafuji T, Tanaka H. Expression pattern of LINGO-1 in the developing nervous system of the chick embryo［J］. Gene Expr Patterns, 2005,6(1):57-62.
［6］Bronner-Fraser M. Analysis of the early stages of trunk neural crest migration in avian embryos using monoclonal antibody HNK-1［J］. Dev Biol, 1986, 115(1): 44-55.
［7］Erickson CA, Duong TD, Tosney KW. Descriptive and experimental anaylysis of the dispersion of neural crest cells along the dorsolateral path and their entry into ectoderm in the chick embryo［J］. Dev Biol, 1992,151(1): 251-272.
［8］Maro GS, Vermeren M, Voiculescu O, et al. Neural crestboundary cap cells constitute a source of Neuronal and glia cells of the PNS［J］. Nat Neurosci, 2004, 7(9):930-938.
［9］Abzhanov A, Tzahor E, Lassar AB, et al. Dissimilar regulation of cell differeniiation in meseneephalic (cranial) and sacral (trunk) neural crest cells in vitro［J］. Development，2003，130(19):4567-4579.
［10］Tessier-Lavigne M, Goodman CS. The molecular bioalogy of axon guidance［J］. Science, 1996, 274(5290): 1123-1133.
［11］Keynes R, Cook GM. Axon guiclance molecule［J］. Cell, 1995, 83(2):161-169.
［12］Dutt S, Kléber M, Matasci M, et al. Versican V0 and V1 guide migratory neural crest cells［J］. J Biol Chem, 2006, 281(17): 12123-12131.
［13］Harris ML, Erickson CA. Lineage specification in neural crest cell pathfinding［J］. Dev Dyn, 2007, 236(1): 1-19.
C2011206032；31100789;省自然科学基金资助项目;国家自然科学基金资助项目
/
| 〈 |
|
〉 |
