目的 通过抑制sox19b基因的表达，探讨sox19b基因在斑马鱼胚胎眼睛发育和形成中的作用。方法 通过显微注射sox19b 吗啉寡聚核苷酸（MO）抑制sox19b 基因的表达，观察胚胎发育过程中表型的变化；采用石蜡包埋组织切片及HE染色、RT-PCR和整封原位杂交等方法探讨敲除sox19b 后对斑马鱼胚胎眼睛发育的调控机制。 结果 敲除sox19b 基因后，斑马鱼胚胎眼睛发育受到影响，表现为眼睛变小或缺失，视网膜及晶状体结构发育异常(n =57/93)；眼睛发育过程中重要调控基因 rx3、pax2a及 vsx2 等表达明显降低，进而影响眼睛的发育和形成。 结论 sox19b 基因作为转录调控因子，可以通过调节眼区转录因子的表达进而影响斑马鱼胚胎早期眼睛的发育和形成。

Objective  To explore the function of sox19b on the eye development in zebrafish by knockdown of sox19b. Methods To inhibit the expression of sox19b by microinjection of sox19b morpholinos（MO）and then observe the effect of the development of the eyes in zebrafish by RT-PCR and the whole-mount in situhybridization. Results The development of the eyes was abnormal after knockdown of sox19b, mainly showed small eyes or eye deficiency and the abnormal retina and lens(n =57/93). The expression of some important genes during the development of the eyes, such as rx3, pax2a and vsx2, was decreased apparently in the sox 19b morphant. Conclusion The transcription factor, sox19b is critical for the development of the eyes via the regulation of the important eye-field transcription factors,EFTEs in early zebrafish embryos.